A kind of tartary buckwheat antioxidant peptide and its preparation method and application

Abstract

The present invention provides a tartary buckwheat antioxidant peptide, the number of which is 3, respectively named tartary buckwheat antioxidant peptide P1, tartary buckwheat antioxidant peptide P2 and tartary buckwheat antioxidant peptide P3, and the amino acid sequences are respectively Gly-Glu-Val ‑Pro‑Trp, Tyr‑Met‑Glu‑Asn‑Phe and Ala‑Phe‑Tyr‑Arg‑Trp with molecular weights of 587.0 Da, 702.8 Da and 741.8 Da, respectively. Also provided is a preparation method of tartary buckwheat antioxidant peptide, which uses tartary buckwheat powder as a raw material to prepare tartary buckwheat albumin powder, and then enzymatically hydrolyzes the tartary buckwheat albumin powder to obtain tartary buckwheat albumin enzymatic peptide, and tartary buckwheat albumin enzymatic peptide Tartary buckwheat antioxidant peptide was isolated and purified. The tartary buckwheat antioxidant peptide prepared by the present invention eliminates the side effects that may be caused by artificially synthesized antioxidants, develops a new use of tartary buckwheat, has the functions of inhibiting lipid peroxidation and scavenging free radicals, and maintains the balance of free radicals in the human body , can be applied in food and medicine, tartary buckwheat antioxidant peptide P3 also has the activity of inhibiting α-glucosidase in vitro, and can be used in the development and utilization of hypoglycemic drugs.

Description

technical field [0001] The invention belongs to the technical field of antioxidant peptides, and in particular relates to a tartary buckwheat antioxidant peptide and a preparation method thereof. Background technique [0002] Tartary buckwheat is a typical embodiment of the homology of medicine and food in my country. It belongs to the Polygonaceae family like "polygonum multiflorum" and "rhubarb". Japan, the United States and Europe and other regions. However, at present, the research on tartary buckwheat is mainly on the physical and chemical properties, while the research on tartary buckwheat protein and biologically active peptides is very little. [0003] Active oxygen and free radicals are substances produced in the normal physiological process of the human body, which can play a variety of functions, such as signal transmission and infection prevention. The human body itself has the ability to remove free radicals, but in some cases, the body cannot remove them in tim...

AI Technical Summary

Problems solved by technology

However, at present, the research on tartary buckwheat is mainly on the physical and chemical properties, while there are few studies on tartary buckwheat protein and bioactive peptides.

[0003] Active oxygen and free radicals are substances produced in the normal physiological process of the human body, which can play a variety of functions, such as signal transmission and infection prevention. The human body itself has the ability to remove free radicals, but in some cases, the body cannot remove them in time Free radicals produced in the body lead to oxidative stress, which may further trigger a series of chronic diseases, such as aging, cancer, cardiovascular disease and other diseases such as atherosclerosis

At present, it has been found that supplementing natural or synthetic antioxidants can be used to prevent a series of damage to biological macromolecules caused by oxidative stress in the body, but synthetic antioxidants have potential risks to health, and their use has begun to be restricted

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