Method for evaluating sensitization of mesenchymal stem cell preparation and application thereof

A technology of mesenchymal stem cells and evaluation methods, which is applied in the field of evaluation of the sensitization of mesenchymal stem cell preparations, can solve the problems of lack of evaluation index technical means for sensitization, and achieve the effect of avoiding inaccurate evaluation results

Inactive Publication Date: 2019-02-15
TIANJIN CHANGHE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a special drug, stem cells lack evaluation indicators and corresponding technical means in the preclinical safety evaluation, especially the evaluation of sensitization

Method used

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  • Method for evaluating sensitization of mesenchymal stem cell preparation and application thereof
  • Method for evaluating sensitization of mesenchymal stem cell preparation and application thereof
  • Method for evaluating sensitization of mesenchymal stem cell preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0060] This example provides a method for evaluating the sensitization of mesenchymal stem cell preparations, evaluating the sensitization of the following two reagents:

[0061] Human umbilical cord mesenchymal stem cell preparation mainly consists of the following components: human umbilical cord mesenchymal stem cells, human serum albumin and physiological saline;

[0062] The umbilical cord mesenchymal stem cell preparation without human source protein mainly consists of the following components: human umbilical cord mesenchymal stem cells and physiological saline.

[0063] experimental design:

[0064] (1) Group settings:

[0065]The mesenchymal stem cell preparations used in this example include human umbilical cord mesenchymal stem cell preparations and umbilical cord mesenchymal stem cell preparations without human source protein. There are 5 groups in the experiment, including: low-dose group: using low-dose human umbilical cord mesenchymal stem cells Animals were i...

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Abstract

The invention provides a method for evaluating the sensitization of a mesenchymal stem cell preparation and the application thereof and relates to the biologically technical field. The method comprises the following steps: immunizing animals by using the mesenchymal stem cell preparation, wherein the immunized animals are divided into a low-dose group and a high-dose group; then dividing the sensitization of the mesenchymal stem cell preparation into preset levels according to symptoms of the immunized animals. The invention further provides a reasonable drug dosage at low and high doses, solves the problem of inaccurate evaluation results caused by unreasonable dosage and alleviates the lack of a method for effectively evaluating the sensitization of the mesenchymal stem cell preparationin the prior art.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and application for evaluating the sensitization of a mesenchymal stem cell preparation. Background technique [0002] Mesenchymal stem cells originate from the mesoderm and ectoderm in the early stages of development and belong to pluripotent stem cells. They have attracted increasing attention because of their characteristics of multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. Mesenchymal stem cells can differentiate into fat, bone, cartilage, nerve, endothelial and other tissue cells under specific induction conditions in vivo or in vitro, and they still have multi-directional differentiation potential after continuous subculture and cryopreservation. Seed cells are used for the repair of tissue and organ damage caused by aging and disease. [0003] According to the data of Cli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/0004
Inventor 徐永胜牟春琳杨莹李伟
Owner TIANJIN CHANGHE BIOLOGICAL TECH
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