Feed additive for improving sea bass liver mitochondria function under high fat stress, preparation method and application thereof, and sea bass feed
A technology of feed additive and seabass liver, which is applied in the field of seabass feed, can solve the problems such as the decline of fish body's anti-stress ability, the slowdown of fish body growth rate, and the economic loss of farmers, so as to improve the antioxidant capacity and reduce oxidative damage , the effect of good application prospects
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[0043] In addition, the present application also provides a preparation method of the above-mentioned feed additive, comprising the following steps: mixing Chinese tallow tree bark extract, rattan tea extract, silymarin, chelated zinc polysaccharide from Dictyophora polysaccharide, methionine chelated iron, biotin and the carrier. Preferably, all raw materials can be pulverized to 150-180 μm before mixing, so as to improve the particle uniformity of solid raw materials and improve the edibility and digestibility of feed additives.
[0044] As a reference, the preparation method of the Chinese tallow tree bark extract can include: extracting the Chinese tallow tree bark with ethanol backflow (the number of backflows can be set to 1 or more times as required), concentrating the extract (when the number of backflows is multiple, The extract to be concentrated is the sum of the extracts collected after each reflux), the extract obtained by concentration is dissolved in water, and ...
Embodiment 1
[0055] Before mixing, all the raw materials are pulverized to 150 μm, and every 100 parts by weight of the feed additive contains 20 parts by weight of Chinese tallow tree bark extract, 10 parts by weight of rattan tea extract, 15 parts by weight of silymarin, and 8 parts by weight of Dictyophora Polysaccharide chelated zinc, 12 parts by weight of methionine chelated iron, 4 parts by weight of biotin, and the balance in the proportion of defatted rice bran are mixed to obtain a feed additive for improving liver mitochondrial function of sea bass under high-fat stress.
[0056] Wherein, the Chinese tallow tree bark extract contains 99% by weight of ellagic acid, and the purity of the ellagic acid is 99.8%. The ligand ratio of methionine chelated iron is 2:1.
[0057] The preparation method of the Chinese tallow tree bark extract comprises: refluxing and extracting the Chinese tallow tree bark with 90wt% ethanol for three times, collecting and combining the extracts after each r...
Embodiment 2
[0061] The difference between this embodiment and Example 1 is that every 100 parts by weight of the feed additive contains 15 parts by weight of Chinese tallow tree bark extract, 15 parts by weight of vine tea extract, 10 parts by weight of silymarin, and 12 parts by weight of Dictyophora Polysaccharide chelated zinc, 9 parts by weight of methionine chelated iron, 6 parts by weight of biotin, and the balance is defatted rice bran.
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