Refrigeration device for collecting of underwater microorganism samples, and underwater microorganism sample collecting device and method

Abstract

The invention discloses a refrigeration device for collecting of underwater microorganism samples, and an underwater microorganism sample collecting device and method. An enrichment device comprises arefrigeration shell and enrichment collecting devices coaxially arranged in the refrigeration shell; the refrigeration shell comprises two oppositely-arranged half shells, a water pipe penetrating hole is formed in the middle of the refrigeration shell, and a cooling device is arranged between the water pipe penetrating hole and the inner wall of the shell; and the enrichment collecting devices are provided with annular clamping buckles, and filter membranes and sand core filters are clamped in the middles of the annular clamping buckles. The underwater microorganism sample collecting devicecomprises a water inlet pipe, a water outlet pipe and one or more enrichment pipelines connected between the water inlet pipe and the water outlet pipe in parallel; and the enrichment pipelines are composed of first valves, the enrichment collecting devices and flowmeters which are connected in series through pipelines. The underwater microorganism sample collecting method comprises the followingsteps that underwater microorganisms are collected through the filter membranes in real time, and the flow and the flow velocity are calculated in real time in the collecting process; and in the collecting process, the filter membranes are cooled in real time, and the cooling temperature is 3-5 DEG C.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a method for collecting and pretreating microorganism samples. Background technique [0002] Due to the widespread use and abuse of antibiotics, the residual antibiotics in the environment exert selective pressure on the microbial community, induce the production of antibiotic resistance genes (ARGs), persist in the environment and spread widely, and have a negative impact on the ecological environment and human beings. Health comes with huge risks. At present, ARGs have become a research hotspot in academia. Pharmaceutical wastewater often contains high concentrations of antibiotics and production raw materials and intermediates, which form a high-intensity selective pressure on the microbial community of the biological treatment unit, providing favorable conditions for the persistent existence and spread of ARGs in sewage. Pharmaceutical wastewater is often discharged ...

AI Technical Summary

Problems solved by technology

The sample collection and pretreatment process is cumbersome and time-consuming, with high requirements for transportation and storage, and the risk of external source contamination is high

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