Double-enzyme cleaning process of cane sugar juice

A cane sugar and process technology, which is applied in the purification of sugar juice, production of sucrose, purification using microorganisms/enzymes, etc., can solve the problems of high production cost, unavailability of enzymes, waste of enzymes, etc., and achieves low cost, reduced dextran content, Economically significant effect

Inactive Publication Date: 2019-02-12
广西糖业集团金光制糖有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the patent involves as many as 4 kinds of enzymes, including dextranase, pectinase, amylase, and cellulase. The optimal reaction temperature and pH value of the above-mentioned 4 enzymes are all different, and they are added to one system at the same time. It will cause some enzymes not to play the corresponding role, resulting in waste of enzymes and high production costs

Method used

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  • Double-enzyme cleaning process of cane sugar juice
  • Double-enzyme cleaning process of cane sugar juice
  • Double-enzyme cleaning process of cane sugar juice

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Add phosphoric acid to the sugarcane mixed juice, and adjust the pH to 7.0 with milk of lime; once heated to 70°C, when the temperature of the mixed juice drops to 55°C, add dextranase to control the concentration of dextranase in the sugar juice to 50ppm, without adding a stirring device, Mix and react during the pumping process. The reaction time is from adding sulfur fumigation to neutralization, and the enzymolysis time is 50 minutes; The reaction temperature is 45°C, adding catechol oxidase, controlling the concentration of catechol oxidase in the sugar juice to 50ppm, stirring the enzymolysis for 30 minutes, then heating to boiling again to terminate the enzyme reaction, sedimentation and filtration to obtain clear juice. The removal rate of dextran is 12%, the filtration speed and sedimentation speed are increased by about 13%, the turbidity is reduced by 26%, the removal rate of phenols is 65%, and the decolorization rate is 42%.

Embodiment 2

[0023] Add phosphoric acid to the sugarcane mixed juice, and adjust the pH to 4.5 with milk of lime; once heated to 60°C, when the temperature of the mixed juice drops to 40°C, add dextranase to control the concentration of dextranase in the sugar juice to 10ppm, without adding a stirring device, Mix and react during the pumping process, the reaction time is from adding to the neutralization of sulfur fumigation, and the enzymolysis time is 15 minutes; after the enzymolysis, carry out sulfur fumigation, the strength of sulfur fumigation is 10CC, add lime milk to adjust the pH value to 5.0, cool To a temperature of 25°C, add catechol oxidase, control the concentration of catechol oxidase in the sugar juice to 10ppm, stir the enzymolysis for 4 minutes, then heat it twice to boil to terminate the enzyme reaction, settle and filter to obtain clear juice. The removal rate of dextran was 42%, the filtration speed and sedimentation speed were increased by about 28%, the turbidity was ...

Embodiment 3

[0025] Add phosphoric acid to the sugarcane mixed juice, and adjust the pH to 6.0 with milk of lime; once heated to 65°C, when the temperature of the mixed juice drops to 50°C, add dextranase to control the concentration of dextranase in the sugar juice to 40ppm, without adding a stirring device, Mix and react during the pumping process, the reaction time is from adding to the neutralization of sulfur fumigation, and the enzymatic hydrolysis time is 40 minutes; after the enzymolysis, sulfur fumigation is carried out, the sulfur fumigation intensity is 13CC, adding lime milk to adjust the pH value to 6.5, and controlling The reaction temperature is 40°C, adding catechol oxidase, controlling the concentration of catechol oxidase in the sugar juice to 40ppm, stirring the enzymolysis for 25 minutes, then heating to boiling again to terminate the enzyme reaction, sedimentation and filtration to obtain clear juice. The removal rate of dextran is 12%, the filtration speed and sediment...

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Abstract

The invention discloses a double-enzyme cleaning process of cane sugar juice, belonging to the technical field of a clarification process of the sugar industry. According to the process, enzymes are added in a cane sugar manufacturing sulfite process for hydrolyzing dextranum in the sugar juice, removing phenol and decoloring; the used enzymes are dextranase and catechol oxidase; the process comprises two stages including the hydrolyzing dextranum as well as removing phenol and decoloring. By means of segmented addition use of the two enzymes, the disadvantages of more varieties of the complexenzymes, less target component pertinence and strict requirements on system temperature are avoided; phenol removal and decoloration are realized while the content of the dextranum is reduced; when the dextranase is added for treating the sugar juice, the dextranum removal rate reaches up to 67%, both of the filtering rate and sedimentation rate are increased by about 56%, the turbidity is reduced to 83%, the phenolic removal rate is as high as 92%, and the decoloration rate reaches up to 87%.

Description

technical field [0001] The invention belongs to the technical field of clarification technology in the sugar industry, and in particular relates to a technology for cleaning sugarcane juice. Background technique [0002] The clarification methods commonly used in domestic sugar refining process are mainly through several ways: 1. Add lime, phosphoric acid, PAM flocculant, SO 2 or CO 2 Precipitation of chemical reagents; 2. Adsorption with activated carbon; 3. Ion exchange. These conventional clarification methods cannot effectively remove the influence caused by such high-viscosity polysaccharide components in sugarcane, the sugar-making material. [0003] At present, it has begun to hydrolyze dextran in the sugar solution by adding glucanase, and a satisfactory effect has been obtained. However, due to the high specificity of the enzyme, a single enzyme preparation cannot effectively eliminate several major polysaccharide components. influences. Catechol oxidase is a ki...

Claims

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Application Information

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IPC IPC(8): C13B20/00C13B20/16
CPCC13B20/002C13B20/16
Inventor 林以宋卢若伟罗凤玉韦红桥陶瑞华
Owner 广西糖业集团金光制糖有限公司
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