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A hafft1 cell

A cell-based and precise technology, applied in the field of HAFFT1 cells and its preparation, can solve problems such as self-protection technology of T cells, and achieve unlimited killing ability, high killing efficiency, and high safety

Active Publication Date: 2020-07-24
BEIJING DCTY BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] None of the above schemes consider the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful tumor microenvironment

Method used

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  • A hafft1 cell
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Examples

Experimental program
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Effect test

Embodiment 1

[0054] This example will take lung cancer patients as an example to provide targeted HAFFT1 cells and their preparation methods:

[0055] 1. Whole Exome Sequencing

[0056] 1) Take peripheral blood from lung cancer patients for ctDNA sequencing and HLA typing;

[0057] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0058] 2. Epitope prediction

[0059] 1) Take the mutated amino acid site as the center, extend 8 amino acids to both sides, and use this 17-amino acid polypeptide as a "potential antigenic epitope";

[0060] 2) Use prediction software to analyze the IC50 of the potential antigenic epitope (recommended software: NetMHCpan 3.0, PickPocket, artificial neural networks (ANN)), if IC50<1000nM, the potential antigenic epitope is considered as "antigenic epitope";

[0061] 3. Synthetic peptides

[0062] Entrust a technical service company to synthesize a m...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a HAFFT1 cell and a preparation method thereof. The cells are modified with TCR-T technology, and the modified T cells are then knocked out of immunosuppressive targets, which precisely protects the specific killer T cells from inhibition in vivo and improves the lethality of T cells against tumor cells . The proportion of specific T cells (TCR+) that recognize tumor antigens in the cells transformed by the HAFFT1 program is over 80%.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a HAFFT1 cell and a preparation method thereof. Background technique: [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proved to be ineffective, while NK, CAR-NK, TIL and other cell technologies have yet to mature. CAR- T cells also have shortcomings in safety and solid tumor therapy. [0003] In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0004] The above treatment methods are immature, especially the in vitro induction of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/90C12N15/867C12N5/10
CPCC12N5/0636C12N15/86C12N15/907C07K14/7051C12N2510/00C12N2740/15043C12N2800/107C12N2810/10C12N9/22C12N2740/16043
Inventor 焦顺昌张嵘张天赋周子珊陈小彬李营营彭刚
Owner BEIJING DCTY BIOTECH CO LTD
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