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Eukaryotic no-reference transcriptome interaction analysis system and method based on computing cloud platform

A technology for interactive analysis and parametric transcriptome, applied in the field of eukaryotic non-parametric transcriptome interactive analysis system, which can solve the problems of personal computers and workstations being unable to complete data processing, low efficiency of manual business processes, etc., to achieve comprehensive content and meet user needs Effect

Inactive Publication Date: 2019-01-18
上海桑格信息技术有限公司
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Problems solved by technology

[0008] In order to solve the above problems, the present invention provides a eukaryotic no-parameter transcriptome interactive analysis system and method based on a computing cloud platform to solve the problems that personal computers and workstations cannot complete data processing and the existing manual business processes are inefficient

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  • Eukaryotic no-reference transcriptome interaction analysis system and method based on computing cloud platform
  • Eukaryotic no-reference transcriptome interaction analysis system and method based on computing cloud platform
  • Eukaryotic no-reference transcriptome interaction analysis system and method based on computing cloud platform

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Embodiment approach

[0093] In one embodiment of the invention:

[0094] For the expression level analysis task in the interactive result analysis module: the quality control result Fastq file can be used as input data, and the fastq file after quality control is based on the newly set parameters, using RSEM or FeatureCount mapping to the sequence of the transcript to obtain New expression analysis results. Alternatively, the count file of the expression analysis result can also be used as input data, and according to the raw count value of each gene generated by the expression analysis, based on the newly set parameters, the differential expression analysis can be performed using DESeq2, DEGseq or edgeR, and then according to the preset The cutoff threshold is used to perform statistics on differential expression results to obtain new differential expression analysis results.

[0095] For the gene structure analysis in the interactive result analysis module: the bam obtained by comparing the qua...

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Abstract

The invention discloses a eukaryotic no-reference transcriptome interaction analysis system and method based on a computing cloud platform. The system comprises a project management module which is used for viewing and managing the project information; a basic analysis task submitting module which is used for setting the basic parameter running task and integrating and encapsulating the result andthe raw data into the corresponding project files according to the preset format; and an interactive result analysis module which is for generating the analysis result according to the user request and visually displaying the result. According to the system and the method, a highly integrated data analysis platform is provided through the help of the cloud computing technology so as to lay a goodfoundation for basic analysis, advanced analysis and personalized analysis of the eukaryotic no-reference transcriptome project.

Description

technical field [0001] The invention relates to the technical field of biological information analysis, in particular to a computing cloud platform-based eukaryotic non-parameter transcriptome interactive analysis system and a method thereof. Background technique [0002] Transcriptome, also known as transcriptome or expression profile, refers to the sum of all RNAs expressed in a certain species, tissue or cell type during a certain period, including protein-coding mRNA and various non-coding RNAs (rRNA, tRNA, snoRNA, snRNA, microRNA, etc.). Transcriptome contains time and space limitations, which is a dynamic connection between genome and external physical characteristics. Transcriptome reflects genes actively expressed under specific conditions, and is an important means to study cell phenotype and function. Transcriptomics is a discipline that studies gene expression changes at the RNA level. For species that do not yet have complete genome sequences, transcriptome sequ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B25/10G16B45/00
CPCG16B25/00G16B45/00
Inventor 刘彬旭余果郭权任一史彩萍曾静石今周玄
Owner 上海桑格信息技术有限公司
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