Method for measuring seaweed sugar concentration in biological matrix

Abstract

Provided is a method for measuring seaweed sugar concentration in biological matrix. The concentration measurement is achieved based on liquid chromatograghy-mass spectrometry combined with pumping-inof auxiliary reagent behind a column. The method comprises the steps that a to-be-measured sample is prepared, specifically, the biological matrix which is quantitatively absorbed is subjected to pretreatment through a protein precipitation method and protein depositions disturbing the measurement of the seaweed sugar concentration are removed; quantitative analysis is carried out, specifically,a to-be-measured sample obtained through a previous treatment is transferred to the chromatograghic column, the auxiliary reagent is pumped behind the column to carry out the liquid chromatograghy-mass spectrometry analysis in a mass spectrometer and a concentration result of nanogram level per milliliter is obtained. According to the method for measuring the seaweed sugar concentration, the situation that the quantitative lower limit of the seaweed sugar quantitative measurement decreases from a microgram per milliliter level to 20 nanogram per milliliter by improving the process of the liquid chromatograghy-mass spectrometry analysis is realized, the sensitivity is significantly improved, the simple and rapid protein precipitation is used to proceed to the pretreatment, processing stepsare apparently simplified, processing time is shortened and the analysis rate is improved.

Description

technical field [0001] The invention relates to a method for analyzing trehalose, in particular to a method for quantitatively measuring the concentration of trehalose in a biological matrix with high precision. Background technique [0002] Trehalose, a non-reducing disaccharide composed of two glucose molecules. Studies have found that trehalose, as a stress metabolite, can effectively protect organisms or some active molecules under harsh environmental conditions such as dehydration, cold, high temperature, etc. These unique biological characteristics make it useful in medicine, It has broad application prospects in food, cosmetics and agriculture. There are many research papers on the preparation of trehalose and its metabolism mechanism in organisms, and with the deepening of research, the determination of trehalose content is a basic premise. Sensitive and rapid determination methods are the key to the accuracy of research and the reliability of results. Guarantees a...

AI Technical Summary

Problems solved by technology

Nevertheless, from the current most preferred analysis method, the quantitative lower limit of the HPLC method is the sensitivity level of micrograms per milliliter, which still cannot meet the needs of analyzing low-concentration samples, and cannot provide a comprehensive and reliable guarantee for the in-depth study of trehalose

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