Method for preparing nutrient concomitant peony seed oil
A kind of technology of peony seed oil and concomitant, applied in the field of preparation of nutritional concomitant peony seed oil
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[0021] The present invention provides a kind of preparation method of nutritive companion peony seed oil, comprising the following steps:
[0022] 1) Enzymolyze the peony seed meal and the compound enzyme solution after mixing to obtain an enzymolyzate;
[0023] The components of the compound enzyme solution include two or more of neutral protease, alkaline protease, cellulase, amylase, pectinase, hemicellulase and glycosidase;
[0024] 2) mixing and homogenizing the enzymolyzate obtained in step 1) with peony seed oil to obtain a homogenate;
[0025] 3) The homogenate liquid obtained in the step 2) is separated from solid to liquid, and the obtained liquid is peony seed oil which is a nutritional companion.
[0026] In the present invention, peony seed meal is mixed with a compound enzyme solution and then enzymolyzed to obtain an enzymatic hydrolyzate; the components of the compound enzyme solution include neutral protease, alkaline protease, cellulase, amylase, pectinase, ...
Embodiment 1
[0042] Weigh compound enzyme (the mass ratio of cellulase and pectinase is 1:1) 100g is dissolved in 2000g water, activate 15 minutes in the constant temperature water bath of 50 ℃, obtain the compound enzyme solution of activation, the compound enzyme solution of activation uniformly mixed with 5000 g of peony seed meal, and subjected to enzymolysis at 50° C. for 6 hours to obtain an enzymolyzate. Weigh 10,000g of peony seed oil (resveratrol content is 0.4μg / mL) and mix it with the enzymatic hydrolyzate, homogenize (2800r / min) enrichment at 50°C for 20min, centrifuge to obtain 8053g of oil, and then The precipitate after centrifugation was press-filtered to obtain 1875g of oil; that is, 9928g of high-nutrition companion peony seed oil was obtained, and the content of resveratrol was 10.21 μg / mL detected by high performance liquid chromatography.
Embodiment 2
[0044] Weigh 1 kg of compound enzyme (the mass ratio of cellulase and pectinase is 1:1) and dissolve it in 40 kg of water, activate it in a jacketed tank at 50° C. for 15 minutes to obtain an activated compound enzyme solution, and dissolve the activated compound enzyme solution uniformly mixed with 50 kg of peony seed meal, and subjected to enzymolysis at 50° C. for 6 hours to obtain an enzymolyzate. Weigh 150kg of peony seed oil (resveratrol content is 0.4μg / mL) and mix it with the enzymatic hydrolyzate, homogenate (2800r / min) at 50°C for 30min, centrifuge to obtain 121.6kg of oil, and then centrifuge The final precipitate was press-filtered to obtain 27.8 kg of oil; that is, 149.4 kg of peony seed oil, a high-nutrition companion, was obtained, and the content of resveratrol was 10.07 μg / mL as detected by high performance liquid chromatography.
[0045] It can be drawn from the above examples that in the nutritional companion peony seed oil prepared by the method provided by...
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