Kit for determining concentration of apolipoprotein C-III and preparation method

An apolipoprotein and kit technology, applied in the biological field, can solve the problems of poor sensitivity and repeatability, and cannot meet the requirements of use, and achieve the effect of improving repeatability and sensitivity

Inactive Publication Date: 2019-01-11
苏州普瑞斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used immunoturbidimetric method for determining the concentration of apolipoprotein C-Ⅲ has a large number of non-specific reactions, and the sensitivity and repeatability of reagent application are poor, which can no longer meet the requirements of use.

Method used

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  • Kit for determining concentration of apolipoprotein C-III and preparation method
  • Kit for determining concentration of apolipoprotein C-III and preparation method
  • Kit for determining concentration of apolipoprotein C-III and preparation method

Examples

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Effect test

Embodiment 1

[0033] A kit for measuring the concentration of apolipoprotein C-III, comprising reagent R1 and reagent R2, the components and concentrations of the reagent R1 include sodium dihydrogen phosphate dihydrate as the first buffer solution A, the concentration of which is 0.8 g / L, as the disodium hydrogen phosphate dodecahydrate of the first buffer solution B, its concentration is 24.31g / L, as the sodium chloride of the first electrolyte, its concentration is 5.8g / L, as the first polymer promoter The PEG 6000 of agent, its concentration is 40g / L, and the Proclin-950 as the first preservative, its concentration is 2.0%, each component and concentration of described reagent R2 include as the second buffer A phosphoric acid dihydrate Sodium dihydrogen at a concentration of 0.5 g / L, disodium hydrogen phosphate dodecahydrate as the second buffer B at a concentration of 23.4 g / L, sodium chloride as the second electrolyte at a concentration of 5.8 g / L L, as the bovine serum albumin of the...

Embodiment 2

[0044] A test kit for measuring the concentration of apolipoprotein C-Ⅲ, comprising reagent R1 and reagent R2, each component and concentration of the reagent R1 including sodium dihydrogen phosphate dihydrate as the first buffer solution A, the concentration of which is 3.2 g / L, as the disodium hydrogen phosphate dodecahydrate of the first buffer solution B, its concentration is 8.75g / L, as the sodium chloride of the first electrolyte, its concentration is 20.0g / L, as the first polymer promoter PEG 6000 as a preservative, its concentration is 80g / L, Proclin-300 as the first preservative, its concentration is 0.8%, the components and concentrations of the reagent R2 include dihydrate phosphate dihydrate as the second buffer A Sodium hydrogen phosphate at a concentration of 2.8 g / L, disodium hydrogen phosphate dodecahydrate at a concentration of 11.5 g / L as the second buffer B, and sodium chloride at a concentration of 20.0 g / L as the second electrolyte , casein as the second s...

Embodiment 3

[0055] A test kit for measuring the concentration of apolipoprotein C-III, comprising reagent R1 and reagent R2, each component and concentration of the reagent R1 including sodium dihydrogen phosphate dihydrate as the first buffer solution A, the concentration of which is 2g / L, disodium hydrogen phosphate dodecahydrate as the first buffer solution B, its concentration is 16g / L, sodium chloride as the first electrolyte, its concentration is 12g / L, PEG as the first polymer accelerator 6000, its concentration is 120g / L, as the thimerosal of the first preservative, its concentration is 1%, and each component and concentration of described reagent R2 comprise as the second buffer solution A dihydrate sodium dihydrogen phosphate, its concentration It is 1.5g / L, as the disodium hydrogen phosphate dodecahydrate of the second buffer solution B, its concentration is 17g / L, as the sodium chloride of the second electrolyte, its concentration is 13g / L, as the second stabilizer Gelatin, i...

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Abstract

The invention relates to the technical field of biology, and particularly to a kit for determining a concentration of an apolipoprotein C-III and a preparation method. The invention adopts the following technical scheme: the kit for determining the concentration of the apolipoprotein C-III is characterized by comprising a reagent R1 and a reagent R2; biotin and streptavidin are added into the reagent R2; the ratio of the biotin to the apolipoprotein C-III antibody is 2:1, and the ratio of the streptavidin to the biotin-apolipoprotein C-III antibody is 1:1. The kit for determining the concentration of the apolipoprotein C-III and the preparation method disclosed by the invention have the advantages that a cascaded amplification reaction is adopted in which the apolipoprotein C-III antibodyis connected with the biotin at the radio of 1:2, and is mixed and reacted with the streptavidin at the radio of 1:1, thereby significantly improving the repeatability and sensitivity, and the linearrange can reach 2-100 mg / L.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a preparation method for measuring the concentration of apolipoprotein C-III. Background technique [0002] Apolipoproteins are generally divided into apolipoprotein A, apolipoprotein B, apolipoprotein C, apolipoprotein D, apolipoprotein E, and apolipoprotein (a), each of which is further divided into several subtypes. The main functions of apolipoprotein are: ① maintain the structure of lipoprotein and lipid transport; ② regulate the activity of enzymes related to lipid metabolism; ③ participate in the metabolism of lipoprotein as a ligand of lipoprotein receptor on the cell surface. Apolipoprotein C (apoC) is a group of water-soluble low-molecular-weight proteins in plasma, including four subclasses: apolipoprotein CⅠ, CII, CIII and CIV, which are mainly distributed in very low-density lipoprotein (VLDL), chyle Microparticles (CM) and high-density lipoprotein (HDL). Apo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 袁嘉扬吴朝晖杜微微李磊
Owner 苏州普瑞斯生物科技有限公司
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