Portable test paper strip for detecting human anti-thyroglobulin antibody and preparation method of portable test paper strip

A portable detection, anti-thyroid technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problem of test strips without TGAb, and achieve the convenience of grass-roots and field use, wide application range, convenient and fast use Effect

Active Publication Date: 2019-01-11
施康培医药科技(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anti-thyroglobulin antibody is expressed to a certain extent in the central lymph node tissue of thyroid cancer. The detection of TGAb level in lymph node tissue can be used as an auxiliary indication for clinical selection of thyroid cancer surgery and postoperative surgical effect. At present, there is no portable rapid TGAb detection test strips

Method used

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  • Portable test paper strip for detecting human anti-thyroglobulin antibody and preparation method of portable test paper strip
  • Portable test paper strip for detecting human anti-thyroglobulin antibody and preparation method of portable test paper strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Preparation of human anti-thyroglobulin antibody

[0036] (1) Preparation of thyroglobulin epitope peptide

[0037] Find the amino acid sequence of human thyroglobulin according to NCBI and other databases, and import the sequence into the epitope design software, count the amino acid sequences located outside the protein conformation, and then import the amino acid sequence of human thyroglobulin into DNASTAR software, and predict the epitope The tool was used to predict, and screened by WesternBlot and Elisa tests, and finally two antigenic epitope peptides with good antigenicity were obtained, and the sequences were respectively shown in SEQ ID NO:1 and SEQ IDNO:2:

[0038] SEQ ID NO:1

[0039] PSCAEGQSCASERQQALSRLYFGTSGYFSQHDLFSSPEKRWASPRVARFATSCPPTIKELFVDSGLLRPMVEGQSQQFSVSENLLAIFPSRG (SEQ ID NO: 1)

[0040] SEQ ID NO:2

[0041] MQYFSHFIRSGNPNYPYEFSRKVPTFATPWPDFVPRAGGENYKEFSELLPNRQGLKKADCSFWSKYISSLKTSADGAKGGQSAESEEEELTAGSGLREDLLSLQEPGSKTYSK (SEQ ID N...

Embodiment 2

[0046] Example 2 Preparation of test strips for detecting human anti-thyroglobulin antibodies

[0047] Such as figure 1 As shown, the test strip for detecting human anti-thyroglobulin antibody includes a bottom plate 7 and a sample pad 1, a binding pad 2, an analysis membrane 3, and a suction pad 6 that are successively built on the bottom plate 7, and the analysis membrane 3 is provided with a detection line. 4 and quality control line 5, the two ends of the detection line 4 and the quality control line 5 are respectively flush with the two sides of the analysis membrane 3, the detection line 4 is set at the end close to the binding pad 2, and the quality control line 5 is set at the end close to the suction sample One end of the pad 6, the colloidal gold-labeled anti-thyroglobulin antibody is bound to the binding pad 2, the detection line 4 is coated with thyroglobulin, and the quality control line 5 is coated with free anti-thyroglobulin antibody. The combined quality cont...

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Abstract

The invention relates to a portable test paper strip for detecting a human anti-thyroglobulin antibody and a preparation method of the portable test paper strip, belonging to the field of immunoassaydetection. The test paper strip comprises a base plate, a sample pad, a binding pad, an analyzing film and a sample suction pad, wherein the sample pad, the binding pad, the analyzing film and the sample suction pad are sequentially butted to the base plate; a detection line and a quality control line are arranged on the analyzing film, and two ends of the detection line and the quality control line are respectively level with two sides of the analyzing film; and a colloidal gold marked anti-thyroglobulin antibody is bound to the binding pad, the detection line is coated with a thyroglobulin antibody, the quality control line is coated with a quality control protein capable of being specifically bound with a free anti-thyroglobulin antibody, and the anti-thyroglobulin antibody correspondsto an epitope peptide of any one of amino acid sequences presented by SEQ ID NO:1-SEQ ID NO:2. The invention further provides a preparation method of the portable test paper strip for detecting the human anti-thyroglobulin antibody. The portable test paper strip is convenient and rapid to use, low in cost and high in specificity and sensitivity.

Description

technical field [0001] The invention relates to the field of immunoassay and detection, more specifically, to a portable test strip for detecting human anti-thyroglobulin antibody and a preparation method thereof. Background technique [0002] Anti-thyroglobulin antibody (TGAb), English name anti-thyroglobulin antibody, is a common autoantibody in the serum of patients with autoimmune thyroid disease. The target antigen of TGAb, thyroglobulin (TG), is a soluble iodinated glycoprotein synthesized and secreted by thyroid epithelial cells, with a molecular weight of 660 kDa and composed of 2748 amino acids. It is the biosynthetic precursor of T3 (triiodothyronine, triiodothyronine) and T4 (tet-raiodothyronine, tetraiodothyronine), and is mainly stored in the thyroid follicle cavity in the form of colloid. Serum content is very small (10-40ng / ml). TGAb is the most typical organ-specific antibody among various human autoantibodies. It is mainly composed of IgG1, IgG2 and IgG4, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/54306G01N33/6854
Inventor 朱富强王珏
Owner 施康培医药科技(武汉)有限公司
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