Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing tumor xenograft model in mice immunized with gastric cancer based on organ-like method, and application thereof

A technology for immunizing mice and organoids, applied in the field of medicine, can solve the problems of difficult to solve drug susceptibility requirements, difficult to raise, and long period of tumor formation.

Inactive Publication Date: 2019-01-11
上海美峰生物技术有限公司
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is based on the "organoid" technology, using microcarriers (NMC1) as a scaffold material, and inoculating human gastric cancer SGC-7901 / MKN-45 cells subcutaneously in normal immune mice to construct an in vivo tumor engineered human gastric cancer xenograft model , so as to solve the problems of nude mice, SCID mice and other immunodeficiency mice in the current human gastric cancer xenograft tumor model, which are difficult to raise, expensive, and low tumor formation rate; especially solve the problem of immunodeficiency mouse human gastric cancer xenograft tumor model It cannot reflect the important role played by the body's immune system in the occurrence and development of tumors, and the immunodeficiency mouse human xenograft tumor model has a long tumor formation cycle (generally takes 4-6 months), it is difficult to solve the urgent need for clinical drugs Treat patients with drug sensitivity needs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing tumor xenograft model in mice immunized with gastric cancer based on organ-like method, and application thereof
  • Method for constructing tumor xenograft model in mice immunized with gastric cancer based on organ-like method, and application thereof
  • Method for constructing tumor xenograft model in mice immunized with gastric cancer based on organ-like method, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0023] Embodiment 1: The method for constructing a human gastric cancer xenograft tumor model in normal immunized mice based on the organoid method of this embodiment is carried out according to the following steps:

[0024] 1) Acquisition of human gastric cancer tumor cells:

[0025] Human gastric cancer cells SGC-7901 / MKN-45 were recovered and added to DMEM complete culture solution containing 10% fetal bovine serum at a temperature of 37°C and 5% CO 2 Cultivate in an incubator, change the medium after 24-48 hours, continue to cultivate, and pass 3-4 times;

[0026] 2) Establish a three-dimensional tumor cell culture model with the organoid method:

[0027] Soak the microcarrier in 75% alcohol by volume for 48 hours, wash it with 1×PBS buffer solution for 5 times, and incubate in DMEM medium for 24-48 hours;

[0028] Take the human gastric cancer tumor cell SGC-7901 / MKN-45 in the exponential growth phase after step 1) passage, if the number of living cells counted by trypa...

specific Embodiment approach 2

[0031] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the volume ratio of the human gastric cancer tumor cell SGC-7901 / MKN-45 suspension and the incubated microcarrier in step 2) is 1:3-5; The optimal value is 1:4. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0032] Embodiment 3: This embodiment differs from Embodiment 1 in that: in step 1), the cells are cultured until the cells grow to a density above 90%, and the cells are subcultured, and the medium is changed every two days. Others are the same as in the first embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing a tumor xenograft model in mice immunized with gastric cancer based on an organ-like method, and an application thereof. The invention relates to a medical tumor animal model, based on an organ-like method. For the first time, the present invention employs a quasi-organ technique, that is, using a microcarrier as substrate. The composite human gastric cancer cell line SGC-7901 / MKN-45 is transplanted subcutaneously into C57BL / 6 mice to establish a tumor model of human gastric cancer with normal immune function. The tumor model constructed by this method has good tumorigenicity, and the tumorigenicity rate is up to 75%. The HE staining and the immunohistochemistry of the transplanted tumor accord with the characteristics of human gastric cancer. Compared with the existing immunodeficient mice transplanted tumor model, the model can better study and further clarify the mechanism of tumor occurrence and development in the normal immune system, and also provides a more valuable new animal model for the research and development of anticancer drugs. The invention is applied to the tumor model field.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a medical tumor animal model based on "organoid" technology, using microcarriers (NMC1) to construct a human gastric cancer normal immune mouse transplanted tumor model, and a method for establishing the model, as well as their use in Applications in tumor research, clinical trials, drug susceptibility testing, and other pharmaceutical fields. Background technique [0002] Tumor is a kind of frequently-occurring and common disease that seriously threatens human health. Its pathogenesis has not been fully elucidated, and the treatment effect is not satisfactory. Existing tumor model research is mainly based on two types, one is the in vitro model, that is, the establishment of two-dimensional and three-dimensional tumor models; the other is the establishment of animal models, mainly human tumor-bearing nude mouse models. [0003] As far as animal models are concerned, the trad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/00A01K67/027
CPCA01K67/02A01K2227/105A01K2267/0331C12N5/0075C12N5/0693
Inventor 洪丰毕研贞潘红娜王全全张凯谌通张凤梅
Owner 上海美峰生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com