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A kind of preparation method of solid bacterial agent for rice crops

A solid inoculum and crop technology, applied in microorganism-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the problem of not being able to simultaneously load biological bacterial fertilizers and chemical fertilizers, fertilizer manpower Waste of material resources, non-interference and other issues

Active Publication Date: 2021-06-22
ZHEJIANG SEGA SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of high fertilizer usage, high frequency of fertilization, serious waste of manpower and material resources in the prior art in the agricultural production process, it is impossible to meet the needs of different fertilizers for crops in different growth periods, and it is impossible to simultaneously load biological fertilizers and chemical fertilizers. And the problem of not interacting with each other provides a solid bacterial agent whose inner layer is placed in a porous gel and whose outer layer is adsorbed with biological bacterial fertilizer

Method used

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  • A kind of preparation method of solid bacterial agent for rice crops
  • A kind of preparation method of solid bacterial agent for rice crops
  • A kind of preparation method of solid bacterial agent for rice crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation steps of solid bacterial agent for rice crops are as follows:

[0037] (1) drying the fertilizer of 35 parts by weight of ammonium sulfate, 20 parts of potassium chloride, 30 parts of superphosphate, 5 parts of magnesium sulfate, and 10 parts of ammonium chloride, and grinding into fine powder;

[0038] (2) 40g of sodium alginate and 25g of polyvinyl alcohol were dissolved in deionized water, and left for 5 hours until fully dissolved to obtain a mixed solution;

[0039] (3) putting the Glomus mosesii strain into the culture medium and cultivating it for 7 days to obtain the bacterial liquid;

[0040] (4) Add the fertilizer fine powder of step (1) and 7g of 1800 mesh CaCO in the resulting mixed solution under stirring 3 ;

[0041] (5) solution gained in step (4) is dripped into 2wt% CaCl 2 In saturated boric acid solution, gel balls are formed after curing for 14 hours;

[0042] (6) Take out the gel ball, put it into a 0.1mol / L hydrochl...

Embodiment 2

[0045] Embodiment 2: the preparation steps of solid bacterial agent for rice crops are as follows:

[0046] (1) drying the fertilizer of 20 parts by weight of ammonium sulfate, 30 parts of potassium chloride, 35 parts of superphosphate, 5 parts of magnesium sulfate, and 10 parts of ammonium chloride, and grinding into fine powder;

[0047] (2) 50g of sodium alginate and 30g of polyvinyl alcohol were dissolved in deionized water, and left for 6 hours until fully dissolved to obtain a mixed solution;

[0048] (3) putting the Glomus mosesii strain into the culture medium and culturing it for 6 days to obtain the bacterial liquid;

[0049] (4) Add the fertilizer fine powder of step (1) and 10g of 800 mesh CaCO in the resulting mixed solution under stirring 3 ;

[0050] (5) solution gained in step (4) is dripped into 3wt% CaCl 2 In saturated boric acid solution, gel balls are formed after curing for 18 hours;

[0051] (6) Take out the gel ball, put it into a 0.1mol / L hydrochlor...

Embodiment 3

[0054] Embodiment 3: the preparation steps of solid bacterial agent for rice crops are as follows:

[0055] (1) drying the fertilizer of 15 parts by weight of ammonium sulfate, 30 parts of potassium chloride, 30 parts of superphosphate, 10 parts of magnesium sulfate, and 15 parts of ammonium chloride, and grinding into fine powder;

[0056] (2) 70g of sodium alginate and 20g of polyvinyl alcohol were dissolved in deionized water, and left for 5 hours until fully dissolved to obtain a mixed solution;

[0057] (3) putting the Glomus mosesii strain into the culture medium and cultivating it for 7 days to obtain the bacterial liquid;

[0058] (4) Add the fertilizer fine powder of step (1) and 15g of 2500 mesh CaCO in the resulting mixed solution under stirring 3 ;

[0059] (5) solution gained in step (4) is dripped into 4wt% CaCl 2 In saturated boric acid solution, gel balls are formed after curing for 19 hours;

[0060] (6) Take out the gel ball, put it into a 0.1mol / L hydroc...

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Abstract

The present invention relates to the field of biological bacterial fertilizer and bacterial agent, in particular to a preparation method of solid bacterial agent for rice crops, comprising the following steps: 1) grinding the fertilizer into fine powder; 2) dissolving sodium alginate and polyvinyl alcohol Fully dissolve in water, add CaCO to the resulting mixture 3 ; Then drop into the cross-linking agent to solidify to form gel balls; 3) Wash the gel balls with an acidic solution to obtain a porous gel material; 4) Graft N-isopropylacrylamide on the surface of the porous gel material ; 5) Mixing and culturing the fungus liquid of Glomus moses and the obtained porous gel material to obtain a solid gel bacterial preparation. The present invention uses sodium alginate / polyvinyl alcohol porous gel material as a carrier, and the pore wall in the porous gel material is covered with fertilizer fine powder, and a large amount of Glomus moses mold is adsorbed on the surface of the pore wall, and the material is Biodegradable materials, will not remain in the soil, green and environmentally friendly.

Description

technical field [0001] The invention relates to the field of biological bacterial fertilizers, in particular to a method for preparing solid bacterial agents for rice crops. Background technique [0002] Rice is one of the crops with the largest planting area and total output in the world, and its output is closely related to world food security. Rice is the staple food for half of the world's population. Rice is a typical mycorrhizal crop. The rice infected by mycorrhizal clusters has good water and nutrient conditions, and shows better growth advantages and resistance compared with rice not inoculated with mycorrhizal clusters. Therefore, it is a kind of scientific research with application prospect to improve the yield of rice with the help of mycorrhizal mycorrhizae. Mycorrhizae—the mutualistic symbiont formed by mycorrhizal fungi and host plants is one of the most common forms of symbiosis in nature, and more than 90% of terrestrial plants have mycorrhizae. Arbuscula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N11/10C12N11/084C12N11/087C12R1/645
CPCC12N1/14C12N11/08
Inventor 胡剑锋胡娟红俞冬冬沈学锋郑锋
Owner ZHEJIANG SEGA SCI & TECH
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