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Method for separating and purifying N-acetylneuraminic acid from material containing polysialic acid

A technology of acetylneuraminic acid and polysialic acid, which is applied in the preparation of sugar derivatives, sugar compounds with non-glycosyl groups, chemical instruments and methods, etc., can solve the problems of high price, complicated process, and few separation and purification methods. , to achieve the effect of low cost, simple steps and meeting market demand

Active Publication Date: 2019-01-11
武汉中科光谷绿色生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many reports on the fermentation methods and techniques for the production of polysialic acid by microbial fermentation, there are not many methods for the separation and purification of products in the downstream stage, and in the only prior art, it is generally to remove the thalline by centrifugation first, and then add A large amount of ethanol and complexes are used to precipitate polysialic acid and protein, and the obtained crude polysialic acid is further obtained after hydrolysis, crystallization, and purification, and ion exchange columns are used in the process. Conducive to industrial production
[0003] At present, market demand areas such as food, health care and medicine have higher quality requirements for N-acetylneuraminic acid products, and the purity is required to be greater than 98%. However, products with a purity greater than 98% are very expensive on the market

Method used

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  • Method for separating and purifying N-acetylneuraminic acid from material containing polysialic acid
  • Method for separating and purifying N-acetylneuraminic acid from material containing polysialic acid
  • Method for separating and purifying N-acetylneuraminic acid from material containing polysialic acid

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Effect test

Embodiment 1

[0029] In this embodiment, the method for separating and purifying N-acetylneuraminic acid from materials containing polysialic acid is as follows:

[0030] 1. Separation of bacterial liquid: use polysialic acid fermentation liquid produced by microbial fermentation as raw material, add polyacrylamide accounting for 15% of the mass of the fermentation liquid for bacterial flocculation and precipitation, and then filter with a ceramic membrane with a pore size of 50nm to make the bacterial The body and the fermentation broth were thoroughly separated to obtain a sterilized fermentation broth, which was detected by high-performance liquid chromatography, and the content of the target product polysialic acid in the fermentation broth had almost no change before and after sterilization;

[0031] 2. Removal of salt ions and miscellaneous proteins: The sterilized fermentation broth is filtered with a 10,000D organic membrane, and a large amount of water is added until the conductivit...

Embodiment 2

[0038] In this embodiment, the method for separating and purifying N-acetylneuraminic acid from materials containing polysialic acid is as follows:

[0039] 1. Separation of bacterial liquid: use polysialic acid fermentation liquid produced by microbial fermentation as raw material, add polyacrylamide accounting for 20% of the mass of the fermentation liquid for bacterial flocculation and precipitation, and then filter with a ceramic membrane with a pore size of 100nm to make the bacterial The body and the fermentation broth were thoroughly separated to obtain a sterilized fermentation broth, which was detected by high-performance liquid chromatography, and the content of the target product polysialic acid in the fermentation broth had almost no change before and after sterilization;

[0040] 2. Removal of salt ions and miscellaneous proteins: The sterilized fermentation broth is filtered with an 8000D organic membrane, and a large amount of water is added until the conductivit...

Embodiment 3

[0047] In this embodiment, the method for separating and purifying N-acetylneuraminic acid from materials containing polysialic acid is as follows:

[0048] 1. Separation of bacterial liquid: use polysialic acid fermentation liquid produced by microbial fermentation as raw material, add polyacrylamide accounting for 25% of the mass of the fermentation liquid for bacterial flocculation and precipitation, and then filter with a ceramic membrane with a pore size of 200nm to make the bacterial The body and the fermentation broth were thoroughly separated to obtain a sterilized fermentation broth, which was detected by high-performance liquid chromatography, and the content of the target product polysialic acid in the fermentation broth had almost no change before and after sterilization;

[0049] 2. Removal of salt ions and miscellaneous proteins: The sterilized fermentation broth is filtered with an 8000D organic membrane, and a large amount of water is added until the conductivit...

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Abstract

The invention discloses a method for separating and purifying N-acetylneuraminic acid from a material containing polysialic acid. The method utilizes a material containing polysialic acid as a raw material, comprises bacterium and solid removal, protein removal, decolorization, hydrolysis, desalting and crystallization and can produce a high-purity N-acetylneuraminic acid product with purity of atleast 98%. The high-purity N-acetylneuraminic acid product can meet the requirements in the fields of foods, health care, medicines and cosmetics. The method is simple and easy to operate and is particularly suitable for industrial fermentation production of N-acetylneuraminic acid.

Description

technical field [0001] The invention relates to a method for separating and purifying N-acetylneuraminic acid from materials containing polysialic acid, and belongs to the technical field of biochemical separation engineering. Background technique [0002] The production of polysialic acid by microbial fermentation has been reported in many literatures. Although there are many reports on the fermentation methods and techniques of polysialic acid produced by microbial fermentation, there are not many methods for the separation and purification of downstream stage products, and in the only prior art, it is generally to remove the thalline by centrifugation first, and then add A large amount of ethanol and complexes are used to precipitate polysialic acid and protein, and the obtained crude polysialic acid is further obtained after hydrolysis, crystallization, and purification, and ion exchange columns are used in the process. Conducive to industrial production. [0003] At p...

Claims

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Application Information

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IPC IPC(8): C07H1/00C07H7/033
CPCC07H1/00C07H7/033
Inventor 袁丽霞陈祥松吴志波吴金勇孙立洁朱薇薇李翔宇王纪王煜王刚费贤春姚建铭
Owner 武汉中科光谷绿色生物技术有限公司
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