Method for establishing Malus sieversii regeneration system based on callus differentiation

Abstract

The invention belongs to the technical field of plant tissue culture, and relates to a method for establishing a Malus sieversii regeneration system based on callus differentiation. The method is completed through the steps of induction of calluses and adventitious buds, proliferation of the adventitious bud, rooting culture, and hardening and transplantation of seedlings. The method allows the callus induction rate to be 100%, the highest induction rate of the adventitious buds to be 30%, the rooting rate to reacj 85% and the survival rate of the seedlings to reach 90%. The method provides abasis for the germplasm preservation and genetic transformation study of Malus sieversii. The method is suitable for various environments, especially Xinjiang, Gansu and other places with low air humidity, greatly improves the survival rate of tissue culture plants, and saves time and labor.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a Xinjiang wild apple regeneration system based on callus differentiation. Background technique [0002] Xinjiang wild apple (Malus sieversii), also known as Seves apple, belongs to the genus Malus and is the ancestor of cultivated apples in the world (Velasco et al.2010; Duan et al.2017). Xinjiang wild apple is a relict species of the Tertiary period, and its fruit shape, coloring, flavor, plant height, tree shape, etc. vary a lot. Because of its self-incompatibility and natural selection, it has formed many unique plants that are cold-resistant, disease-resistant, and poor-resistant. The rich variation has won widespread attention and has become the main rootstock in Northwest China and other provinces. one. The Tianshan wild fruit forest located in the Ili Valley of Xinjiang, with wild apples as the main species, is an impor...

AI Technical Summary

Problems solved by technology

However, little research has been done on the tissue culture system of the ancestral species of cultivated apple, Xinjiang Wild Apple.

Li Xinyue (2018), Liu Bing (2011), Tan Dongmei (2009) established the tissue culture system of Xinjiang wild apple with stem segments as explants, but the transformation efficiency was low; (2017) used the leaves of Xinjiang wild apple 31 as explants to directly induce adventitious buds, but this material is a "highly renewable material" selected (Li Baisha, 2014), and the leaves can be induced directly. produce adventitious buds; while the frequency of in vitro regeneration of wild apples in Xinjiang largely depends on the genotype of the test material, most wild apple germplasm cannot directly induce adventitious buds, therefore, it is necessary to find a more universal and optimized Method for obtaining adventitious buds based on leaf callus differentiation

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