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Application of orange leaf extract in preparation of medicine for treating/preventing autoimmune orchitis

A technology for autoimmunity and orchitis, applied in the field of biomedicine, can solve problems such as unclear active parts, and achieve the effect of improving the utilization value

Active Publication Date: 2022-03-18
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Orange leaves are flat, bitter, pungent, non-toxic, and belong to the liver meridian. clear

Method used

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  • Application of orange leaf extract in preparation of medicine for treating/preventing autoimmune orchitis
  • Application of orange leaf extract in preparation of medicine for treating/preventing autoimmune orchitis
  • Application of orange leaf extract in preparation of medicine for treating/preventing autoimmune orchitis

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] The preparation method of orange leaf extract is:

[0031] (1) Ethanol extraction: the dried orange leaf sample is crushed, extracted 3-6 times with ethanol with a mass concentration of 80-90%, and the dosage is 5-15 times the mass volume (kg / L) of the dried orange leaf sample , 1-7 days each time. concentrating the extract under reduced pressure until it has no alcohol smell to obtain an ethanol extract, and concentrating the ethanol extract under reduced pressure and evaporating to dryness to obtain an ethanol extract;

[0032] (2) Macroporous resin elution: Ethanol extracted extract was diluted with water, passed through D101 macroporous resin, eluted with double distilled water, ethanol and acetone in sequence, and the eluted parts of ethanol and acetone were concentrated and combined;

[0033] (3) Extraction and concentration: Suspend the combined concentrate with water, extract with cyclohexane, ethyl acetate and n-butanol in sequence, extract 3-6 times with each...

Embodiment 1

[0042] Preparation of orange leaf extract

[0043] (1) 17kg of dried orange leaf samples were crushed, extracted 5 times by cold immersion in 85L of 85% ethanol, concentrated under reduced pressure until 5.58L of extract (A) was obtained, and the extract (A) was placed in a 60°C water bath Heat and stir evenly, take 100mL of extract, concentrate under reduced pressure and evaporate to dryness to obtain 43.5g of ethanol extract (C), and 5.58L of extract is converted to obtain 2.43kg of ethanol extract (C).

[0044] (2) Ethanol extraction extract (A) was diluted with water, passed through D101 macroporous resin, eluted with double distilled water, 95% industrial ethanol and acetone in sequence, concentrated under reduced pressure to recover the eluted part of 95% industrial ethanol and acetone, and concentrated The objects were combined to obtain (B) after TLC plate control.

[0045] (3) Suspend the concentrate (B) with water, and extract 5 times with cyclohexane, ethyl acetate a...

Embodiment 2

[0047] The method is the same as in Example 1, except that the mass concentration of ethanol in step (1), the amount of ethanol used for each extraction, the number of cold-dipping extractions, and the time of each cold-dipping extraction; the amount of water and D101 macroporous resin used in step (2), The amount of redistilled water, ethanol and acetone used for eluting, the mass concentration of ethanol; the amount of suspension water used in step (3), the amount of cyclohexane, ethyl acetate and n-butanol, and the number of extraction times of each solvent; the corresponding obtained extract The quality is also different. See Table 1 for detailed data:

[0048] The preparation of table 1 orange leaf extract

[0049]

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Abstract

The invention belongs to the technical field of biomedicine and discloses the application of orange leaf extract in the preparation of medicine for treating / preventing autoimmune orchitis. Using the leaves of citrus which is rich in resources in my country as raw material, the citrus leaf ethanol extract obtained by ethanol cold soaking is then eluted by macroporous resin, and the concentrate of ethanol and acetone eluted parts is combined, dispersed in water, and successively used After extraction with cyclohexane, ethyl acetate and n-butanol, it was concentrated. Pharmacological experiments show that the orange leaf extract has anti-autoimmune orchitis effect, and can be developed into a drug for treating / preventing autoimmune orchitis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of orange leaf extract in preparing medicine for autoimmune orchitis. Background technique [0002] Infertility has become a global medical and social problem. At present, about 15% of couples of childbearing age in the world suffer from infertility, of which about 50% are caused by male factors. With the postponement of the average childbearing age and the full liberalization of the two-child policy, the problem of infertility in my country has become increasingly prominent. According to the "China Infertility Status Survey Report" jointly issued by the China Population Association and the National Family Planning Commission in 2012, the infertility rate of China's childbearing population has climbed from 2.5%-3% 20 years ago to 12.5%-15 %, the number of patients has exceeded 40 million, that is, one in every eight couples has infertility problem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/752A61P15/08A61P29/00A61K127/00
CPCA61P15/08A61P29/00A61K36/752A61K2236/333A61K2236/55A61K2236/51A61K2236/39
Inventor 董发武何红平饶高雄李应仙张鑫睿赵成美李芳
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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