Method for detecting and analyzing active sites of anti-pulmonary fibrosis of Periplaneta americana L.

Abstract

The invention belongs to the technical field of traditional Chinese medicine fingerprint analysis, and discloses a method for detecting and analyzing active sites of anti-pulmonary fibrosis of Periplaneta americana L.. The method comprises the following steps of: B, constructing a ML-HB fingerprint, constructing a spectrum-activity relationship regression equation by combining chromatographic peakarea values of the ML-HB fingerprint using partial least squares method, and selecting a common peak positively correlated with pharmacological activity in the chromatographic peak of the ML-HB of the active sites of anti-pulmonary fibrosis of the Periplaneta americana L.. According to the method for detecting and analyzing the active sites of anti-pulmonary fibrosis of the Periplaneta americanaL., the HPLC-ELSD fingerprint of the ML-HB of the active sites of anti-pulmonary fibrosis of the Periplaneta americana L. and the spectrum-activity relationship inhibiting the growth of HEL cells thereof are established, and a relationship between information in a chemical fingerprint and a pharmacological activity can be better reflected compared to a simple chemical component fingerprint, so that a new idea is provided for a quality control study of the ML-HB.

Description

technical field The invention belongs to the technical field of traditional Chinese medicine fingerprint analysis, and in particular relates to a method for analyzing and detecting the drug effect of the active part of the American cockroach against pulmonary fibrosis. Background technique At present, as a comprehensive and quantifiable identification method based on the chemical composition information of traditional Chinese medicine, the fingerprint of traditional Chinese medicine has become an internationally recognized method for controlling the quality of natural medicines and proprietary Chinese medicines. It can well match the characteristics of traditional Chinese medicine and achieve The purpose of quality control and analysis of traditional Chinese medicine. However, the research and establishment of fingerprints only improve the stability and controllability of Chinese medicine ingredients, and have no direct internal connection with the efficacy of Chinese medici...

AI Technical Summary

Problems solved by technology

However, in the prior art, the relationship between its material basis and drug efficacy has not been well analyzed, so that it is impossible to conduct a good study on the anti-pulmonary fibrosis active site ML-HB of Periplaneta americana

Images