Preparation method of macrocarpal C

A technology of eucalyptus aldehyde and mellow taste, applied in the field of chemistry, can solve the problems of no preparation method, such as eucalyptus aldehyde, and achieve the effect of high purity

Inactive Publication Date: 2018-12-28
淮安安莱生物科技有限公司
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Problems solved by technology

[0005] At present, there is no simple and mature preparation method i

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  • Preparation method of macrocarpal C
  • Preparation method of macrocarpal C
  • Preparation method of macrocarpal C

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Embodiment Construction

[0049] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.

[0050] 1. Experimental materials

[0051] The leaves of blue eucalyptus were collected from the back mountain of Heilongtan in the northern suburb of Kunming, Yunnan.

[0052] AB-8 macroporous adsorption resin was purchased from Tianjin Haoju Resin Technology Co., Ltd.

[0053] High-speed countercurrent chromatography TEB300B was purchased from Shanghai Tongtian Biotechnology Co., Ltd.

[0054] 2. Experimental methods and results

[0055] Including the following steps:

[0056] Step S1, extraction: collect Eucalyptus globulus leaves, dry in the shade, pulverize, extract with a volume fraction of 75% ethanol aqueous solution under heat reflux, the solid-to-liquid ratio is 1:20, extract 3 times, each time for 1.5h, filter, and combine the filtrates to concentrate to No a...

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Abstract

The invention discloses a preparation method of macrocarpal C. The method comprises the following steps: Eucalyptus globulus leaves are collected, dried in the shade and smashed, heat reflux extraction is performed with ethanol water with volume fraction being 75%, extraction is performed multiple times, filtering is performed, and filtrates are mixed and concentrated until no alcohol smell; the extract concentrated to be free of alcohol smell is loaded to an AB-8 macroporous adsorption resin column for enrichment, 10-12BV eluate of 70% ethanol is collected, concentrated until no alcohol smelland freeze-dried, and freeze-dried powder of an enriched product is obtained; the freeze-dried powder of the enriched product is ultrasonically dissolved in an isovolumetric top phase and bottom phase mixed solvent for HSCCC separation and purification, n-butyl alcohol/ethyl acetate/tetrahydrofuran/water in the volume ratio being 5:18:4:18 is selected as an HSCCC two-phase solvent system, elutionfraction corresponding to the macrocarpal C is collected according to a chromatogram, concentrated and dried, and the macrocarpal C is obtained. The preparation method is simple, industrial production is easy, and the prepared macrocarpal C is high in purity.

Description

technical field [0001] The invention belongs to the field of chemistry, and relates to a preparation method of macrocarpal B and macrocarpal C. Background technique [0002] Macrocarpal B and macrocarpal C are natural products present in Eucalyptus eucalyptus leaves, and their chemical structures are as follows: [0003] [0004] Studies have found that macrocarpal B and macrocarpal C have excellent anti-tumor activity (Chinese patents 2018110395176, 2018110400136, 2018110400193), and have the prospect of being developed into anti-tumor drugs. [0005] At present, there is no simple and mature preparation method in the prior art for the preparation of macrocarpal B and macrocarpal C. Contents of the invention [0006] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a preparation method of macrocarpal B and macrocarpal C. [0007] The above object of the present invention is achieved through the following technical solu...

Claims

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Application Information

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IPC IPC(8): C07C45/78C07C47/57
CPCC07C45/78C07C2603/30C07C47/57
Inventor 郑嘉荣万超吕学金
Owner 淮安安莱生物科技有限公司
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