Preparation method of surface imprinted particles capable of efficiently separating flavone molecules

Abstract

The invention discloses a preparation method of surface imprinted particles capable of efficiently separating flavone molecules. 50-100 mL of N,N-dimethylformamide, 5-10 g of rutin template moleculesand 5-15 mL of MAA (methyl acrylic acid) are added to a container and react for 5-10 min under magnetic stirring, 1-3 g of macroporous resin and 1-3 g of a crosslinking agent are added, the containeris sealed, continuous stirring and nitrogen introduction are performed for 5 min, then, the container is placed in a constant-temperature water bath at 30-50 DEG C, 0.05-0.2 g of an initiator is addedto the container, and the mixture reacts for 10-15 h under the continuous stirring condition; product particles are filtered and separated, the particles are eluted repeatedly by an eluent and washedby distilled water until no flavone molecules are detected in a washing liquid, and then, vacuum drying is performed to obtain the surface imprinted particles capable of efficiently separating flavone molecules. The prepared imprinted particles have specific recognition selectivity and higher adsorption capacity, and can efficiently separating and purifying flavone.

Description

technical field [0001] The invention relates to a microparticle and a preparation method thereof, in particular to a preparation method of surface imprinted microparticles capable of efficiently separating flavonoid molecules. Background technique [0002] With the continuous improvement of people's living standards, the development of active ingredients in natural medicines and plants is becoming more and more popular. However, natural plants not only have a relatively low content of active ingredients and are not easy to enrich, but also contain a wide variety of compounds in the system, the system is complex, macromolecules and small molecules, living and non-living substances coexist, especially the existence of molecules with similar structures, leading to separation Purification is difficult. In addition, the active ingredients of many natural products are sensitive to heat and easily hydrolyzed, making separation and purification difficult. [0003] At present, the ...

AI Technical Summary

Problems solved by technology

However, these traditional separation materials have poor selectivity and are not easy to separate components with similar structures, resulting in low purity of the extracted drugs and difficulty in ensuring drug efficacy. This requires multiple or multi-stage separations to obtain active components with high purity, but These traditional separation materials have low separation efficiency and recovery rate, and will cause environmental pollution

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