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Multilayer device for separating blood components and uses thereof

A lower layer, support layer technology, applied in the field of separation of liquid sample components, can solve problems such as low yield and lack of fully automated analysis

Active Publication Date: 2018-12-21
PARTNERSHIP FOR CLEAN COMPETITION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More specifically, there is a need for a product and technology that utilizes a simplified sample collection process, reduced cost, and simplified shipping and storage that overcomes the need for centrifugation of whole blood components (Sturm et al., Bioanalysis 2015, 7, 1987 -2002 ("Sturm"); Kim et al., Anal Chem 2013, 85, 11501-11508 ("Kim"); Li et al., Rapid Commun Mass Spectrom 2012, 26, 1208-1212 ("Li")), present Narrow hematocrit range available for testing, i.e. Hct 40-55% (Sturm), low yield of plasma volume (Sturm; Kim; Li), and lack of fully automated analysis (Kim; Li)

Method used

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  • Multilayer device for separating blood components and uses thereof
  • Multilayer device for separating blood components and uses thereof
  • Multilayer device for separating blood components and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0159] Structure of a book-type multi-level device

[0160] To prepare filter membrane units, such as RBC filter discs, Membrane filter uses HARRIS Uni-Core with 5mm diameter TM A hole punch (Amazon, USA) was used to punch holes. The bottom support layer comprising 5 mm diameter notched discs was prepared using an OSBORNE arch punch (Amazon, USA) using a cardstock material similar to the cap or top support layer. Such as figure 1 As shown, after preparing each layer of material, by first placing the polyester hydrophobic film layer (4) on the inner side of the underside of the top cover card material (2), and then punching four through the upper card structure equidistantly. Assemble the card with a base or top cover and holes through the polyester hydrophobic membrane layer (4). The bottom raised or raised support (6) is made from two discs of lid support cardstock material secured to the inner surface of the bottom lid cardstock material (7) using an adhesive material su...

example 2

[0164] Automated flow-through elution coupled to online SPE-LC-MS / MS bioanalysis of blood for opioids and stimulants

[0165] To design, develop and validate a hematocrit-capable multilayer device capable of producing plasma without centrifugation, suitable for automated on-line liquid chromatography with tandem mass spectrometry detection (LC / MS / MS) analysis, a A multilayer device is used to prepare dried plasma spot samples from whole blood microsampling. Extraction of the resulting dried plasma spot was achieved by direct elution followed by online solid-phase extraction (SPE) and LC / MS / MS determination of the analytes of interest, in this example opioids and stimulant. Four opioids and five stimulants with varying physicochemical properties were selected for testing in this analysis.

[0166] A series of standard working solutions were prepared by diluting the primary stock solution with 3:7 methanol / water (v / v). Calibration standards were also prepared at 5 ng / mL, 10 n...

example 3

[0225] Test Compounds for Testing Multilayer Devices

[0226] The multilayer device of the present invention was tested using a known hypertension and ADHD drug, guanfacine, which has [ 13 C, 15 N 3 ] Internal standard. Guanfacine (C 9 h 9 Cl 2 N 3 O) has a monoisotopic mass of 245.0123 Da, while the internal standard [ 13 C, 15 N 3 ]-guanfacine ( 13 CC 8 h 9 Cl 2 15 N 3 O) has a monoisotopic mass of 249.0067 Da. These compounds were tested using whole blood samples with a blood:plasma binding ratio (Ke / p) of 1.5 and analyzed using LC-MS / MS bioanalysis following the protocol described herein.

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Abstract

Embodiments of the invention are generally directed to analyte detection and products facilitating the collection, separation of sample components, and analyte detection. The multilayer device that allows for a rapid, easy, accurate, and efficient test of a fluid sample for analytes of interest and methods of collecting, separating components, and testing using the multilayer device are describedin various embodiments of the invention.

Description

[0001] Cross References to Related Applications [0002] This international PCT application claims the benefit of priority to U.S. Provisional Patent Application No. 62 / 299226, filed February 24, 2016, entitled "Dried Plasma Spot Card For Automated Online Determination Of Opioids," which is incorporated by reference in its entirety incorporated into this application. technical field [0003] The present invention generally relates to the separation of liquid sample components for analyte detection. More specifically, aspects of the invention relate to a simple and accurate device for separating various components of whole blood using a multilayer device for separating blood components or a multilayer separation device, and the use of such a device to detect, for example, A method for analytes in various blood components on the surface of cells and in intracellular and extracellular fluids, wherein the various components of whole blood include, but are not limited to, red bloo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40G01N33/48G01N33/49G01N33/50G01N33/52
CPCG01N2001/288G01N2001/4088G01N33/491G01N1/4077G01N33/48B01L3/5023B01L2300/0681B01L2300/0816B01L2300/165G01N33/56966
Inventor 约翰·D·亨利安伊梅尔达·瑞娜拉瑞·D·鲍尔斯
Owner PARTNERSHIP FOR CLEAN COMPETITION
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