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Ptps-based vaccines against cancer

A technology of PTP and vaccine, applied in the field of prior translation product, immune response, preferably immune response against tumor antigen, vaccine composition, composition containing said PTP

Inactive Publication Date: 2018-12-21
INSTITUT GUSTAVE ROUSSY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, accumulating evidence suggests that tumor-derived exosomes are not perfect, as they may induce tumor immune evasion through different roles in different pathways, such as by inhibiting DC differentiation or by negatively regulating NK cells (Valenti, Huber et al., 2006; Clayton, Mitchell et al., 2008; Whiteside, Mandapathil et al., 2011)

Method used

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  • Ptps-based vaccines against cancer
  • Ptps-based vaccines against cancer
  • Ptps-based vaccines against cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0099] Example 1 - Combination of advanced translation products (PTPs) and exosomes: a novel cancer vaccine.

[0100] Materials and Methods

[0101] cell culture

[0102] at 37°C and 5% CO 2 Under standard conditions, the MCA 205 mouse sarcoma cell line was grown in RPMI 1640 medium (Life Technologies) in the presence of 1% glutamine, 1% pyruvate, 1% non-essential amino acids and 10% FBS (Life Technologies) under cultivation. at 37°C and 5% CO 2 B16F10 (syngeneic cells from C57BL / 6J mice) were cultured in DMEM containing 10% FCS, 2 mM L-glutamine and 100 IU / ml penicillin / streptomycin.

[0103] MCA 205 and B16F10 cells were transfected with the YFP-globin-intron-SL8-his plasmid using JetPrime according to the manufacturer's protocol for PTP purification (Ozyme). For tumor rejection experiments, stable MCA 205-Ova and stable B16F10-Ova cells were prepared. Stable MCA 205-Ova was grown in RPMI 1640 under standard conditions. Stable B16F10-Ova cells stably expressing oval...

Embodiment 2

[0137] Example 2 - Vaccine against melanoma

[0138] PTP-based vaccines against melanoma:

[0139] The inventors showed in Example 1 that PTP purified from sarcoma cell lines such as MCA205 can be used as a vaccine in mice to elicit specific anti-tumor T cell responses in a prophylactic manner. To expand the inventors' view that PTPs are useful as vaccines against cancer, they investigated other types of cancer. For this purpose, the inventors purified PTPs from melanoma cell lines such as the murine B16F10 cell line. Next, different groups of 6 mice were vaccinated with different concentrations of PTP, with the adjuvant itself (negative control) or with the SL8 epitope emulsified in the same adjuvant (positive control). These PTPs were purified from a mouse B16F10 tumor cell line previously transfected with our globin-intron-SL8-His construct. After 2 weeks, 50.10 4 The cells were injected subcutaneously into the right flank of mice, and the transfected B16F10 tumor cell ...

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Abstract

The present invention relates to the field of medicine. It more particularly relates to peptides, microvesicles containing such peptides, compositions containing same, in particular vaccine, and methods for stimulating an immune response in a subject.

Description

technical field [0001] The present invention relates to the field of medicine and is generally used in the field of therapy and prevention. The present invention relates more particularly to products of prior translation ("PTPs") consisting of peptides having 7 to 50 amino acids, to microvesicles containing such PTPs, to compositions, in particular vaccine compositions, containing said PTPs, And to a method of stimulating an immune response in a subject, preferably an immune response against a tumor antigen. Background technique [0002] The main goal of vaccination is to induce effective immune responses that can control viral infectious diseases and cancer in humans. The immune system is divided into two categories: on the one hand the innate immune system and on the other hand the adaptive immune system. The cellular immune response against infected or transformed cells requires the activation of the adaptive immune system. This activation can only be achieved by stimu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00
CPCA61K39/00A61K39/0011A61P35/00A61K2039/585A61K2039/545C07K14/70539C07K7/08A61K2039/876A61K39/00119C07K7/06A61K2039/53A61K2039/5152
Inventor 塞巴斯蒂安·阿普谢罗宾·福雷乌斯山坂享弘艾莉森·皮尔森玛蒂尔德·鲍尔皮坎特
Owner INSTITUT GUSTAVE ROUSSY
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