A method for measuring the metabolic energy of plant leaf cells

A technology for plant leaf and cell metabolism, which is applied to measuring devices, instruments, scientific instruments, etc., and can solve problems such as unclear demand and supply of metabolic energy

Active Publication Date: 2020-11-13
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although the internal energy charge state is currently used to reflect the metabolic energy of cells in organisms, in fact, the demand and supply of metabolic energy for the assimilation and dissimilation of many substances are not clear, and the demand and supply of metabolic energy for many metabolic processes are also unclear. , therefore, only measuring the state of energy charge in cells cannot truly represent the metabolic energy of plant cells
[0005] In addition, even if the state of intracellular energy charge can reflect the metabolic energy of cells in an organism, the state of intracellular energy charge of the living body is also required, which is difficult to achieve with existing technologies

Method used

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  • A method for measuring the metabolic energy of plant leaf cells
  • A method for measuring the metabolic energy of plant leaf cells
  • A method for measuring the metabolic energy of plant leaf cells

Examples

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Embodiment

[0106] Take Baiji as an example. In the base of Puding Karst Ecological Comprehensive Experimental Station of Guizhou Province, Chinese Academy of Sciences, biennial white and plants were picked, and they were quickly returned to the laboratory. After cleaning the surface dust of the leaves on the fresh branches, the leaves to be tested were collected from the fresh branches one by one, and put Soak in distilled water for 30 minutes; blot the water on the surface of the leaf, immediately clamp the leaf to be tested between the parallel electrode plates of the measuring device, set the measuring voltage and frequency, and set different clamping forces by changing the quality of the iron block, parallel mode The physiological capacitance, physiological resistance, and physiological impedance of plant leaves under different clamping forces were measured; the physiological capacitance of white and different leaf positions under different clamping forces is shown in Table 1, the phy...

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Abstract

The invention discloses a method for measuring plant leaf cell metabolic energy, and belongs to the technical field of biophysical information detection. A measuring device comprises a bracket, foam plates, electrode plates, wires, an iron block and a plastic rod, wherein the foam plates embedded with the electrode plates are separately adhered to the bottom end of the bracket and the plastic rod.The electrode plates are connected with an LCR tester through the wires during using, the two electrode plates clamp a plant leaf to be measured, and physiological resistance, physiological impedanceand physiological capacitance of the plant leaf under different clamping force are simultaneously measured in a parallel mode. According to the Gibbs free energy equation and the Nernst equation, models of the physiological capacitance of the plant leaf changing with the clamping force, the physiological resistance of the plant leaf changing with the clamping force and the physiological impedanceof the plant leaf changing with the clamping force are further constructed, and the plant leaf cell metabolic energy is calculated jointly by using parameters of the three models.

Description

technical field [0001] The invention belongs to the technical field of biophysical information detection, and specifically relates to a method for measuring the metabolic energy of plant leaf cells, which can quickly and non-destructively detect the metabolic energy of plant leaf cells, and judge the supply relationship of water and substances in the plant leaf system. Background technique [0002] "Energy" is classically defined as the ability of an object to do work. Energy is a measure of the motion of matter, corresponding to different motion forms of matter, energy also has different forms. Life activities are the sum of sequence reactions leading to a highly ordered state of the system, and consume energy. Although different organisms can use different sources of energy; however, in reality a large number of biochemical reactions and cellular processes in the body can only accept metabolic energy, the form of energy that is directly available for the metabolism of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/04G01N27/22
CPCG01N27/041G01N27/22
Inventor 吴沿友吴沿胜方蕾吴明开王瑞苏跃王世杰刘从强
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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