Continuous preparation method for antibody (or antigen)-coated microspheres

A technology of microspheres and antibodies, which is applied in the preparation of microspheres, microcapsule preparations, measuring devices, etc., can solve the problems of low utilization of antibody antigens, uneven microsphere packaging, and large differences in microspheres. The effect of small size, good effect and high production efficiency

Inactive Publication Date: 2018-12-18
NANAN CHUANGPEI ELECTRONICS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under such operating conditions, completely uniform mixing between the two phases is not easy to achieve, and the microenvironment around each microsphere may vary greatly, which directly leads to the difference between the coated microspheres. Large, large product differences between batches of coating reaction production, low utilization rate of antibodies (or antigens), etc.
And before mixing the microspheres and the antibody (or antigen), the microspheres and the antibody (or antigen) should be dispersed very evenly, otherwise the microspheres are prone to agglomeration after the antibody is labeled, resulting in uneven coating of the microspheres

Method used

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  • Continuous preparation method for antibody (or antigen)-coated microspheres
  • Continuous preparation method for antibody (or antigen)-coated microspheres

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Embodiment 1

[0025] The present invention provides a continuous preparation method of antibody (or antigen) coated microspheres. The specific preparation steps are as follows:

[0026] Step 1: First prepare microspheres, antibody or antigen solution, condensing agent and buffer;

[0027] Step 2: Inject the microspheres in the microsphere tank 3 and the condensing agent in the condensing agent tank 4 into the first tank 1 through the first pressure pump and pipeline for mixing, and the antibody or antigen solution in the antibody or antigen solution tank 5 The buffer solution in the buffer solution tank 6 is injected into the second tank body 2 through the first pressure pump and the pipeline for mixing;

[0028] Step 3: The first tank body 1 and the second tank body 2 are equipped with a first air inlet pipe 7, which is operated by a fan 17, and the gas is blown into the gas distribution pipe 13 through the main inlet pipe 16 and heated by the heating layer 14, and then passes through the first ...

Embodiment 2

[0034] The present invention also provides figure 1 A continuous preparation device for antibody (or antigen) coated microspheres is shown. The top of the first tank 1 is connected with a microsphere tank 3 and a condensing agent tank 4 through a pipeline, and a pressure pump is provided on the pipeline , The top of the second tank 2 is connected with an antibody or antigen solution tank 5 and a buffer tank 6 through a pipe, and the first tank 1 and the second tank 2 are both provided with a first air inlet pipe 7, The inner side of the first tank body 1 and the second tank body 2 is provided with a header pipe 8, the header pipe 8 is provided with a second pressure pump, the end of the header pipe 8 is fixedly provided with a shunt pipe 9, and the bottom of the shunt pipe 9 There is a collecting branch 10, the top of the collecting branch 10 is provided with a filter tank 11, and a filter membrane 12 is provided inside the filter tank 11.

[0035] Further, in the above technical...

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Abstract

The invention discloses a continuous preparation method for antibody (or antigen)-coated microspheres. The method comprises the specific preparation steps that first, microspheres, an antibody or antigen solution, a condensing agent and a buffer solution are prepared; and second, the microspheres in a microsphere tank and the condensing agent in a condensing agent tank are injected into a first tank body through a first pressure pump and a pipeline for mixing, and the antibody or antigen solution in an antibody or antigen solution tank and the buffer solution in a buffer solution tank are injected into a second tank body through the first pressure pump and the pipeline for mixing. According to the method, by introducing hot air through air inlet pipes in the liquid mixing process, liquid mixing is more uniform, and mixed liquid can be heated to improve the speed and efficiency of microsphere coating by antibodies or antigens, so that the microspheres are better coated; and moreover, the processing steps are simple, continuous preparation can be realized, and the method can be suitable for large-batch production.

Description

Technical field [0001] The present invention relates to the field of antibody (or antigen) coated microspheres, in particular to a continuous preparation method of antibody (or antigen) coated microspheres. Background technique [0002] The process of coating (adsorbing) antibodies or antigens onto microspheres can be divided into two categories. One is called the physical adsorption method. The basic process is to mix the antibody or antigen with the microspheres to be coated in a suitable buffer system, and use the interaction force between the antibody or antigen and the surface of the microspheres to produce adsorption. The molecules are fixed on the surface of the microspheres to realize the process of coating the microspheres. Another method is called chemisorption. The basic process is to use some active groups (such as carboxyl, amino, hydroxyl, sulfhydryl, etc.) on the surface of the microspheres and the corresponding groups (amino, carboxyl, sulfhydryl) on macromolecul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543B01J13/02
CPCG01N33/531B01J13/02G01N33/54313
Inventor 王玉环
Owner NANAN CHUANGPEI ELECTRONICS TECH CO LTD
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