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Test strip for rapid detection for transgene product

A test strip and genetically modified technology, applied in the direction of measuring devices, microbiological determination/inspection, instruments, etc., can solve the problems of time-consuming, high requirements for experimental facilities and conditions, and achieve the effect of fast and simple detection and easy discrimination and analysis

Inactive Publication Date: 2018-12-18
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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Problems solved by technology

However, the PCR method requires high experimental facilities and conditions, and is time-consuming.

Method used

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  • Test strip for rapid detection for transgene product
  • Test strip for rapid detection for transgene product
  • Test strip for rapid detection for transgene product

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Experimental program
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Embodiment Construction

[0043] Below, the test strip for rapid detection of genetically modified products of the present invention will be described in detail in conjunction with the accompanying drawings.

[0044] The test strip for rapid detection of transgenic products of the present invention comprises in sequence: a sample pad, a gold standard binding pad, a nitrocellulose membrane detection area and a water-absorbing pad;

[0045] The colloidal gold-labeled Digoxigenin antibody is coated on the gold-labeled binding pad;

[0046] The nitrocellulose membrane detection area is provided with a detection line and a quality control line; the detection line is formed by coating streptavidin on the nitrocellulose membrane detection area; the quality control line passes through the nitrocellulose membrane detection area Coated with goat anti-mouse IgG;

[0047] The reaction product of the product to be detected is dropped onto the sample pad and then chromatographed toward the absorbent pad through cap...

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Abstract

The invention discloses a test strip for rapid detection for a transgene product. The test strip comprises a sample pad, a colloidal-gold combination pad, a nitrocellulose membrane detection zone andan absorbent pad; the colloidal-gold combination pad is coated with a colloidal gold-labeled digoxin antibody; the nitrocellulose membrane detection zone is provided with a detection line and a quality control line; the detection line is formed by coating streptavidin on the nitrocellulose membrane detection zone; the quality control line is formed by coating goat anti-mouse IgG on the nitrocellulose membrane detection zone; a reaction product of the product to-be-detected is dropped onto a sample pad, and then is chromatographed in the direction of the absorbent pad through capillary action.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and in particular relates to a test strip for rapidly detecting transgenic products. Background technique [0002] General transgenic detection is divided into nucleic acid level detection and protein level detection. [0003] Protein level detection (existing colloidal gold protein test paper technology) is convenient for detection, but because some transgenic sample proteins are not expressed and the protein is easily degraded, it is possible that the protein expression in the sample to be tested may not be expressed or the storage conditions of the sample to be tested may cause degradation. Therefore, false negative results are caused (positives are not detected). The protein test strip method has the advantages of rapidity, simplicity, and economy, and does not require any special equipment. However, due to its low detection sensitivity and narrow application range, it is main...

Claims

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Application Information

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IPC IPC(8): C12Q1/6804G01N33/558
CPCC12Q1/6804G01N33/558C12Q2531/113C12Q2565/625C12Q2563/131
Inventor 李雨峰陈立柱杨海侠刘亚赵久然任雯
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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