Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of gene gmmyb32 encoding soybean myb32 transcription factor

A MYB32, transcription factor technology, applied in the field of genetic engineering, can solve the problem of incomplete analysis of the regulation mechanism of phosphorus element

Active Publication Date: 2022-02-11
NANJING AGRICULTURAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mechanism of plant regulation of phosphorus is still not fully resolved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of gene gmmyb32 encoding soybean myb32 transcription factor
  • Application of gene gmmyb32 encoding soybean myb32 transcription factor
  • Application of gene gmmyb32 encoding soybean myb32 transcription factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0043] Genetic engineering application of embodiment 2 gene GmMYB32

[0044] 1) Cloning of soybean MYB32 transcription factor encoding gene GmMYB32

[0045] Using the root total RNA of soybean (Glycine max) phosphorus-sensitive varieties Silver and Honeycomb Bean 73 as templates, the first strand of cDNA was synthesized by reverse transcription, and PCR amplification was performed. The primer sequences are shown in SEQ ID NO.3 and SEQ ID NO. 4. The PCR program is as follows: pre-denaturation at 95°C for 3 minutes, denaturation at 95°C for 15 seconds, annealing at 60°C for 15 seconds, extension at 72°C for 1 minute, a total of 35 cycles, and a final incubation at 72°C for 5 minutes, followed by constant temperature at 12°C. The product was cloned into the pUC19-TVector vector, and after sequencing, the CDS sequence of the soybean GmMYB32 gene with a length of 1017 bp and a complete coding region was obtained, wherein the sequence of the coding region is shown in SEQ ID NO.1;

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of soybean MYB32 transcription factor coding gene GmMYB32. The soybean MYB32 transcription factor coding gene GmMYB32 has a nucleotide sequence of SEQ ID NO.1. The constructed plant overexpression vector pMDC83‑GmMYB32 was heterologously expressed in the wild type of Arabidopsis thaliana, and it was found that the overexpressed plants had enhanced growth and biomass under low phosphorus conditions, and the main root length and shoot fresh weight were higher than those of the wild type. The contrast increased significantly, indicating that the gene can be introduced into plants as a target gene, and by enhancing plant growth, reversing the phosphorus deficiency symptoms of plants, and improving the ability of transgenic plants to tolerate low phosphorus. It can be seen that the soybean MYB32 transcription factor encoding gene GmMYB32 of the present invention can be used in enhancing plant growth through genetic engineering, reversing the phosphorus deficiency symptoms of plants, and improving the ability of transgenic plants to tolerate low phosphorus.

Description

technical field [0001] The invention relates to the application of soybean MYB32 transcription factor encoding gene GmMYB32, which belongs to the field of genetic engineering, and specifically relates to the enhancement of plant growth and biomass by the MYB32 transcription factor encoding gene derived from soybean, thereby enhancing the low phosphorus tolerance and yield of transgenic plants aspects of application. Background technique [0002] Phosphorus is one of the three essential elements for plants. Insufficient phosphorus supply will have adverse effects on plant growth and metabolism. The actual phosphorus concentration in the soil is far from meeting the needs of plants, and phosphorus fertilizers are usually required to supplement the phosphorus supply. However, the utilization rate of phosphorus fertilizer by crops is seriously insufficient. At present, the mechanism of plant regulation of phosphorus is still not fully resolved. Studying the function of genes ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/54A01H6/20A01H6/00A01H6/82
CPCC12N15/8261C12N15/8273C07K14/415
Inventor 程浩赵梦杨宇明王晴喻德跃
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com