Neural crest-lineage pericyte derived from multi-potential stem cells and induced differentiation method of neural crest-lineage pericyte

Abstract

The invention discloses a neural crest-lineage pericyte derived from multi-potential stem cells and an induced differentiation method of the neural crest-lineage pericyte. The induced differentiationmethod comprises the following steps: dissociating the multi-potential stem cells to obtain single-cell suspension, inoculating the single-cell suspension into a matrigel-coated pore plate, and changing the suspension through an NCN2 culture medium each day; carrying out culturing for a certain period of time, carrying out digestion, and collecting cells; carrying out adherent culture on the collected cells, changing culture liquid into pericyte induced culture liquid, carrying out subculture, and detecting the cell phenotype of the pericyte. According to the induced differentiation method, the disadvantages of limited source of human cerebral vessel pericytes, and the heterogeneity and linkage heterogeniety of the pericytes derived from multi-potential stem cells obtained in other non-finite induction ways are solved, and the obtained neural crest-lineage pericyte has very strong blood vessel stabilizing capacity and contractility; and by virtue of a standardized induced differentiation procedure, cell populations obtained in different batches have good coherence.

Description

technical field [0001] The invention belongs to the field of biology and medicine, and more specifically, the invention relates to a neural crest lineage pericyte derived from pluripotent stem cells and a method for inducing differentiation thereof. Background technique [0002] Pericytes, also known as Rouget cells or vascular wall cells, cover the outside of microvascular endothelial cells and exist in capillaries, postcapillary venules and precapillary arterioles. In recent years, the function of pericytes has attracted more and more attention. It plays an important role in the morphogenesis and function of blood vessels, cardiovascular homeostasis, and diseases such as ischemic stroke and diabetic retinopathy. However, there is no clear definition of pericytes at present. As a compromise, it is usually judged by comprehensive location, morphology, and gene / protein expression patterns. [0003] Pericytes are ubiquitous in blood vessels. About 80% of microvessels in the b...

AI Technical Summary

Problems solved by technology

[0010] However, the pericytes obtained by the methods reported in these literatures or patents are either differentiated from mesoderm, or the differentiation path is not controlled, and only focus on solving the source of pericytes, and do not provide corresponding solutions for the different developmental origins of pericytes. Therefore, the induced cells may be highly heterogeneous; at the same time, these solutions do not focus on the function of the cells, and products with poor therapeutic effects will greatly limit their application

Images