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Microbial remediation method of heavy-metal-contained waste residue stockpiling site

A microbial remediation and heavy metal technology, applied in the restoration of contaminated soil, etc., can solve the problems of slow heavy metal removal speed, short storage time, low plant survival rate, etc., and achieve the effect of optimizing large-scale cultivation, high efficiency, and quick results

Active Publication Date: 2018-11-23
SCI RES ACADEMY OF GUANGXI ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phytoremediation is also a promising heavy metal pollution remediation technology at present, but the difficulty of phytoremediation lies in: ① due to the chemical conditions and heavy metal toxins that are not suitable for plant survival, the survival rate of plants is low, the storage time is short, and it is difficult to establish a sustainable plant ecosystem
②Slow heavy metal removal rate and incomplete heavy metal metabolism limit the application of phytoremediation
It should also be emphasized that the above methods are only aimed at sites that have been polluted by heavy metals (no new sources of heavy metal pollution), and are difficult to apply to the treatment of polluted sites where heavy metals are continuously leached out (hazardous waste storage sites)

Method used

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  • Microbial remediation method of heavy-metal-contained waste residue stockpiling site

Examples

Experimental program
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Effect test

Embodiment 1

[0037] a. Acidophilic bacteria and sulfate-reducing bacteria were collected from the soil of tailing ponds of Guangxi nonferrous metal beneficiation enterprises;

[0038] b. Crush sweet sorghum stalks and place them in a fermenter, mix them with lactic acid bacteria at a ratio of 200:1, add water and stir evenly, keep the water at 50%, and ferment in a sealed container for 3 days for later use;

[0039]c. After the fermentation is completed, take out the sweet sorghum stalk fermentation liquid, add magnesium sulfate heptahydrate 0.4g / L, ammonium sulfate 1.2g / L, potassium dihydrogen phosphate 0.6g / L, ferrous sulfate heptahydrate 0.4g / L , slowly and intermittently add sodium hydroxide to adjust the pH to 6, the preparation of the medium is completed, and the medium is placed in an anaerobic culture bottle for high-pressure steam sterilization, the sterilization pressure is 105kPa, the temperature is 118°C, and the sterilization is 25min;

[0040] d. Mix the soil contaminated by ...

Embodiment 3

[0057] a. Acidophilic bacteria and sulfate-reducing bacteria were collected from the soil of tailing ponds of Guangxi nonferrous metal beneficiation enterprises;

[0058] b. Crush sweet sorghum stalks and put them in a fermenter, mix them with lactic acid bacteria at a ratio of 250:1, add water and stir evenly, keep the water at 40%, and ferment naturally for 4 days for later use;

[0059] c. After the fermentation is complete, take out the fermented sweet sorghum stalk, add magnesium sulfate heptahydrate 0.5g / L, ammonium sulfate 1.0g / L, potassium dihydrogen phosphate 0.5g / L, ferrous sulfate heptahydrate 0.5g / L , slowly and intermittently add sodium hydroxide to adjust the pH to 7, the preparation of the medium is completed, and the medium is placed in an anaerobic culture bottle for high-pressure steam sterilization with a sterilization pressure of 103kPa and a temperature of 121°C for 20 minutes;

[0060] d. Mix the soil polluted by heavy metal waste slag with the culture me...

Embodiment 4

[0067] a. Acidophilic bacteria and sulfate-reducing bacteria were collected from the soil of tailing ponds of Guangxi nonferrous metal beneficiation enterprises;

[0068] b. Crush sweet sorghum stalks and place them in a fermenter, mix them with lactic acid bacteria at a ratio of 250:1, add water and stir evenly, keep the water at 50%, and ferment naturally for 4 days for later use;

[0069] c. After the fermentation is complete, take out the fermented sweet sorghum stalk, add magnesium sulfate heptahydrate 0.5g / L, ammonium sulfate 1.1g / L, potassium dihydrogen phosphate 0.5g / L, ferrous sulfate heptahydrate 0.5g / L , slowly and intermittently add sodium hydroxide to adjust the pH to 7, the preparation of the medium is completed, and the medium is placed in an anaerobic culture bottle for high-pressure steam sterilization with a sterilization pressure of 105kPa and a temperature of 120°C for 20 minutes;

[0070] d. Mix the soil polluted by heavy metal waste slag with the culture ...

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Abstract

The invention discloses a microbial remediation method of a heavy-metal-contained waste residue stockpiling site. Hazardous wastes containing heavy metal of lead, zinc, cadmium and the like are used for serving as culture media, sulfate reducing bacteria are subject to domestication, the survival capacity and the environment adaptation of the sulfate reducing bacteria in the hazardous wastes containing heavy metal of lead, zinc, cadmium and the like are improved, various kinds of nutrient substances are added to enable the sulfate reducing bacteria and bacillus subtilis to be mixed to reinforce restoration of the soil, the pollutant is rapidly removed, and the soil crop planting capacity is restored.

Description

technical field [0001] The invention relates to a restoration method for heavy metal waste slag storage site and surrounding soil pollution, belonging to the field of environmental protection. Background technique [0002] Attention should be paid to the pollution remediation of the waste residue storage sites containing cadmium, lead, zinc and other heavy metals and the surrounding areas. Due to the production and disposal of some hazardous wastes, the pollution prevention measures of enterprises are not in place, resulting in the leakage and scattering of these typical hazardous wastes during the long-term storage and transfer process, causing the site and surrounding soil to be polluted, and even the groundwater to be polluted. The cost of repairing the polluted site is huge, and ordinary enterprises cannot afford it. Therefore, the polluted site will exist for a long time, and the pollutants will continue to spread with the precipitation and rain, causing a larger area o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C2101/00
Inventor 赵侣璇刘凯覃楠钧张立宏宋晓薇樊勇吉徐荣乐谢祎敏
Owner SCI RES ACADEMY OF GUANGXI ENVIRONMENTAL PROTECTION
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