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Preparation method of earthworm protein microsphere nano-trauma complex and application

A technology of ground dragon protein and compound, which is applied in the direction of hydrolyzed protein components, peptide/protein components, medical preparations containing active ingredients, etc., and can solve the problems of not having the conditions of drug sustained-release materials

Active Publication Date: 2018-11-20
SHAANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are superparamagnetic nanoparticles SPIO (superparamagnetic iron oxidenanoparticles) commercial products (Endorem, Feridex, Resovist), these are ferric oxide nanoparticles covered by polysaccharides, and the particle size of the whole particle is 50nm, but these None of the materials have the conditions to be used as drug sustained-release materials

Method used

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  • Preparation method of earthworm protein microsphere nano-trauma complex and application
  • Preparation method of earthworm protein microsphere nano-trauma complex and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of ground dragon protein microsphere nano-wound complex, comprising the following steps:

[0023] S1. Preparation of earthworm protein: Wash fresh earthworm three times with normal saline to remove body surface sediment and dirt, crush it with a Constant pressure crusher to obtain cell crushing slurry, add to the cell crushing slurry Physiological buffer (Tris-HCl, 20mM, pH=7.5) was centrifuged at 4°C for 5 minutes at a low speed to obtain a mixture of damaged cells after removing the sediment and digested matter in the body; prepare 10%~ 70% gradient sucrose solution, lightly place the cell damage mixture on the upper end of the sucrose solution, centrifuge at 2500rpm, collect the upper layer component, middle layer component, and lower layer component in the centrifuge tube respectively, and then pass the conventional ion exchange method (with NaCl 1M, Tris-HCl 20mM cation elution) and gel chromatography (using 20mM Tris-HCl as eluent) to prepare ...

Embodiment 2

[0031] The ground dragon protein microsphere nano-wound complex prepared in Example 1 can be used for animal wound healing.

example

[0033] 4-week-old Balb / C mice were used for the experiment. After adaptively feeding the mice for 1 week, they were divided into 3 groups named: blank group (Blank), positive control group (JWH), and earthworm nano group (EW). conduct experiment. Anesthetized by intraperitoneal injection of 10% chloral hydrate, the experimental mice were placed in a prone position, placed on a sterile drape, and the skin on the back was disinfected with iodine.

[0034] Then perform mechanical depilation on the back of the mouse, use sterile ophthalmic scissors to make a circular incision (2 cm in diameter) on the back to create an open wound model, and put it into a cage to continue raising.

[0035] Apply medicine to the mouse wound according to the wound area, and the dosage is 100mg / cm 2 (calculated by the amount of protein), the blank group was only smeared with normal saline, the positive control group was smeared with commercial drugs, and the earthworm nano group was smeared with the ...

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PUM

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Abstract

The invention discloses a preparation method of an earthworm protein microsphere nano-trauma complex and application. The preparation method comprises the following steps: preparing earthworm proteins, preparing carbon nanospheres, preparing transition system metal hollow nanospheres, and preparing the earthworm protein microsphere nano-trauma complex. Compared with the prior art, the preparationmethod disclosed in the invention has the advantages that the proteins are embedded into the transition system metal hollow nanospheres in a physical mode, so that a medical pharmaceutical preparationwith sustained-release, induced and multisite effects can be prepared, and the aim of promoting trauma repair is achieved.

Description

technical field [0001] The invention relates to the technical field of protein drug preparation, in particular to a preparation method and application of a ground dragon protein microsphere nano-wound compound. Background technique [0002] In modern society, the number of patients with various traumas caused by traffic accidents, accidental events (burns, mechanical injuries, etc.), medical operations, etc. is constantly increasing. Studies have shown that the death rate due to trauma ranks fourth among various diseases worldwide. According to statistics, 100,000 people die every year due to trauma in my country, and 1 million people are disabled, causing direct economic losses of 10 billion yuan. With the development of society, this number is still rising. Therefore, post-traumatic repair has always been a hot topic in the medical field. In recent years, studies have found that the traditional Chinese medicine Dilong (earthworm, Lumbricus) has shown a unique curative e...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K9/50A61K47/02A61P17/02A61K35/62
CPCA61K9/501A61K35/62A61K38/012A61P17/02
Inventor 王东杨欢刘念权珍妮王瑞辉
Owner SHAANXI UNIV OF CHINESE MEDICINE
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