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Homocysteine detection kit with strong anti-interference ability

A technology of homocysteine ​​and detection kits, which is applied in the field of clinical medical detection and can solve problems such as result deviation

Inactive Publication Date: 2018-11-16
济南贝韩生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a homocysteine ​​diagnostic detection kit with strong anti-interference ability. The kit of the present invention focuses on solving the problem of result deviation caused by interfering substances in the detection process

Method used

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  • Homocysteine detection kit with strong anti-interference ability
  • Homocysteine detection kit with strong anti-interference ability
  • Homocysteine detection kit with strong anti-interference ability

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0054] Reagent R1 consists of:

[0055] Serine 0.1mol / L

[0056] NADH 30mmol / L

[0057] LDH 25KU / L

[0058] GOODS buffer 0.1mol / L

[0059] Mercaptoethanol 30 mmol / L

[0060] Trion X-405 1ml / L

[0061] Chloroform 5ml / L

[0062] VC oxidase 3KU

[0063] EDTA-Na 2 50mmol / L

[0064] BSA 12g / L

[0065] Dextran 2000 5g / L

[0066] Mannitol 7.5g / L

[0067] Potassium sorbate 2.5g / L

[0068] The composition of reagent R2:

[0069] Cystathionine β-synthase 5KU / L

[0070] Cystathionine β-decomposing enzyme 5KU / L

[0071] Triethanolamine buffer 0.05mol / L

[0072] Dextran 2000 5g / L

[0073] Mannitol 7.5g / L

[0074] Potassium sorbate 2.5g / L

[0075] Trion X-405 4ml / L

[0076] Glutathione 2g / L

example 2

[0078] Serine 0.1mol / L

[0079] NADH 30mmol / L

[0080] LDH 25KU / L

[0081] GOODS buffer 0.1mol / L

[0082] Mercaptoethanol 30 mmol / L

[0083] Trion X-405 1ml / L

[0084] Chloroform 4ml / L

[0085] VC oxidase 4KU

[0086] EDTA-Na 2 40mmol / L

[0087] BSA 12g / L

[0088] Dextran 2000 7.5g / L

[0089] Mannitol 10g / L

[0090] Potassium sorbate 5g / L

[0091] The composition of reagent R2:

[0092] Cystathionine β-synthase 5KU / L

[0093] Cystathionine β-decomposing enzyme 5KU / L

[0094] Triethanolamine buffer 0.05mol / L

[0095] Dextran 2000 7.5g / L

[0096] Mannitol 10g / L

[0097] Potassium sorbate 5g / L

[0098] Trion X-405 4ml / L

[0099] Glutathione 2g / L

example 3

[0101] Serine 0.1mol / L

[0102] NADH 30mmol / L

[0103] LDH 25KU / L

[0104] GOODS buffer 0.1mol / L

[0105] Mercaptoethanol 30 mmol / L

[0106] Trion X-405 1ml / L

[0107] Chloroform 3ml / L

[0108] VC oxidase 3KU

[0109] EDTA-Na 2 40mmol / L

[0110] BSA 12g / L

[0111] Dextran 2000 7.5g / L

[0112] Mannitol 10g / L

[0113] Potassium sorbate 5g / L

[0114] The composition of reagent R2:

[0115] Cystathionine β-synthase 5KU / L

[0116] Cystathionine β-decomposing enzyme 5KU / L

[0117] Triethanolamine buffer 0.05mol / L

[0118] Dextran 2000 7.5g / L

[0119] Mannitol 10g / L

[0120] Potassium sorbate 5g / L

[0121] Trion X-405 4ml / L

[0122] Glutathione 2g / L

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Abstract

The invention relates to a homocysteine diagnostic kit with strong anti-interference ability. The kit is a liquid type double-reagent diagnostic test kit, and is composed of a reagent R1 and a reagent R2. Four anti-interference substances which are chloroform, VC oxidase, mercaptoethanol and EDTA-Na2 are introduced, so the performances of the kit are qualitatively improved, and the kit has a strong stability and strong anti-interference ability. The homocysteine detection kit with strong anti-interference ability has a high marketing advantage and a good application value.

Description

technical field [0001] The kit of the invention relates to a homocysteine ​​detection kit with strong anti-interference ability. The kit of the invention relates to the field of clinical medical detection, and mainly relates to the detection of homocysteine ​​in human serum. Background technique [0002] Homocysteine ​​is a sulfur-containing amino acid that is an important intermediate product in the metabolism of methionine and cysteine. Studies have shown that homocysteine ​​can cause damage to vascular endothelial cells by producing oxides, thereby changing the function of blood coagulation factors. Therefore, when the function of blood coagulation factors is changed, it will increase the possibility of thrombus formation, promote atherosclerosis, and increase the incidence of vascular diseases. Therefore, the clinical diagnosis and detection of homocysteine ​​is of great significance for the prevention and diagnosis of vascular diseases. [0003] Commonly used methods ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6815
Inventor 李建营胡晓飞罗维晓李静张培培
Owner 济南贝韩生物技术有限公司
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