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Compound enzyme preparation for reducing TSNAs (tobacco-specific nitrosamines) as well as preparation method and application of compound enzyme preparation

A compound enzyme preparation and nitrosamine technology, which is applied in the field of tobacco leaf processing, can solve the problems of high preparation cost of microbial inoculants, restricting large-scale popularization and application, and large influence of environmental conditions, so as to reduce the content of TSNAs and facilitate large-scale popularization. Apply and improve the effect of quality and safety

Active Publication Date: 2018-11-16
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of microbial agents in the control of TSNAs content in tobacco leaves has limitations in the following aspects: (1) The fermentation and preparation process of microbial agents requires repeated optimization of a large number of experimental parameters, and the fermentation and preparation processes are also time-consuming (2) The preparation cost of microbial agents is relatively high; (3) The colonization ability, growth and metabolic activity of microorganisms on tobacco leaves, and environmental conditions have a greater impact on their functions. The above problems limit the Large-scale popularization and application of microbial agents in TSNAs content control
There are few reports on reducing the content of TSNAs in tobacco leaves during curing by enzyme treatment

Method used

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  • Compound enzyme preparation for reducing TSNAs (tobacco-specific nitrosamines) as well as preparation method and application of compound enzyme preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The compound enzyme preparation for reducing tobacco leaf-specific nitrosamines in this embodiment is composed of the following components by mass percentage: 0.03% neutral protease, 0.02% pectinase, 0.01% xylanase, 0.02% glucoamylase, β- Glucanase 0.01%, sterile distilled water 99.91%.

[0028] The preparation method of the above-mentioned compound enzyme preparation that present embodiment relates to, comprises the steps:

[0029] (1) Prepare raw materials according to the above components;

[0030] (2) Stir and mix the components of step (1) and sterile distilled water according to the mass percentage, the stirring speed is 75rpm, the mixing temperature is 40°C, and the pH is 5.5, until the compound enzyme preparation suspension is uniformly obtained, that is Compound enzyme preparation.

[0031] The application of the above-mentioned compound enzyme preparation involved in this embodiment in the preparation of sun-cured tobacco includes the following steps:

[00...

Embodiment 2

[0039] The compound enzyme preparation for reducing tobacco leaf-specific nitrosamines in this embodiment is composed of the following components by mass percentage: 0.02% neutral protease, 0.01% pectinase, 0.005% xylanase, 0.01% glucoamylase, β- Glucanase 0.005%, α-amylase 0.01%, medium temperature amylase 0.02%, cellulase 0.02%, sterile distilled water 99.90%.

[0040] The preparation method of the above-mentioned compound enzyme preparation that present embodiment relates to, comprises the steps:

[0041] (1) Prepare raw materials according to the above components;

[0042] (2) Stir and mix the components of step (1) and sterile distilled water according to the mass percentage, the stirring speed is 50rpm, the mixing temperature is 35°C, and the pH is 5.2, until the compound enzyme preparation suspension is uniformly obtained, that is Compound enzyme preparation.

[0043] The application of the above-mentioned compound enzyme preparation involved in this embodiment in the...

Embodiment 3

[0049] The composite enzyme preparation for reducing tobacco leaf-specific nitrosamines of this embodiment consists of the following components in mass percentage:

[0050] Tobacco compound enzyme 0.5%, neutral protease 0.01%, pectinase 0.005%, xylanase 0.0025%, glucoamylase 0.005%, β-glucanase 0.0025%, α-amylase 0.005%, medium temperature amylase 0.005% %, cellulase 0.005%, neutral lipase 0.01% and sterile distilled water 99.45%.

[0051] The preparation method of the above-mentioned compound enzyme preparation that present embodiment relates to, comprises the steps:

[0052] (1) Prepare raw materials according to the above components;

[0053] (2) Stir and mix the components of step (1) and sterile distilled water according to the mass percentage, the stirring speed is 100rpm, the mixing temperature is 45°C, and the pH is 5.8, until the compound enzyme preparation suspension is uniformly obtained, that is Compound enzyme preparation.

[0054] The application of the above-...

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Abstract

The invention relates to a compound enzyme preparation for reducing TSNAs (tobacco-specific nitrosamines) as well as a preparation method and an application of the compound enzyme preparation. The compound enzyme preparation comprises sterile distilled water as well as five or more of neutral protease, pectinase, xylanase, glucoamylase, beta-glucanase, alpha-amylase, medium-temperature amylase, cellulase, neutral lipase and tobacco complex enzyme. After treatment with the compound enzyme preparation, content of TSNAs in yellow sun-cured tobacco is reduced by 26.40%-36.81%. The compound enzymepreparation is low in cost and convenient to operate, is easily popularized and applied on a large scale and has great application value in harm decrease of raw materials of tobacco, and quality safety of the tobacco is improved.

Description

technical field [0001] The invention relates to a compound enzyme preparation, in particular to a compound enzyme preparation for reducing tobacco leaf-specific nitrosamines, and also relates to a preparation method and application thereof, belonging to the field of tobacco leaf processing. Background technique [0002] The use of modern biotechnology to degrade the harmful components and precursors of tobacco leaves is an important way to reduce the harm of cigarettes. In recent years, with people's increasing attention to the relationship between smoking and health, tobacco-specific nitrosamines (Tobacco-specific nitrosamines, referred to as TSNAs) in tobacco have attracted much attention because of their potential carcinogenicity, and have become a tool for tobacco companies to reduce the harm of tobacco. One of the important research contents. TSNAs mainly include four harmful substances: N-nitrosonornicotine (NNN for short), 4-(N-methyl-nitroso)-1-(3-pyridyl)-1-butanon...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N9/18C12N9/26C12N9/88C12N9/24C12N9/42C12N9/34C12N9/20A24B3/12
CPCA24B3/12C12N9/18C12N9/20C12N9/2408C12N9/2411C12N9/2414C12N9/2428C12N9/2437C12N9/2448C12N9/248C12N9/50C12N9/88C12Y301/01003C12Y301/01011C12Y302/01001C12Y302/01003C12Y302/01015C12Y302/01073C12Y402/02002
Inventor 米其利高茜夭建华刘巍管莹陆舍铭李雪梅徐玉琼钱颖颖朱洲海
Owner CHINA TOBACCO YUNNAN IND
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