Quadruple probiotic solid beverage and preparation method thereof
A technology of quadruple probiotics and solid beverages, applied to bacteria, lactobacillus, bifidobacteria and other directions used in food preparation, can solve the problems of uneven product quality and effect, ensure the number of viable bacteria, reduce harmful Bacteria, the effect of enhancing immunity
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Embodiment 1
[0051] Embodiment 1: A kind of quadruple probiotic solid beverage and its preparation method
[0052] 1. Formula
[0053] All raw materials are obtained from commercially available qualified raw and auxiliary materials that meet the corresponding national standards / industry standards and national regulations after strict screening. Accurately weigh 8kg of Lactobacillus rhamnosus freeze-dried powder, 5kg of Lactobacillus reuteri freeze-dried powder, 5kg of Bifidobacterium animalis freeze-dried powder, 5kg of Bifidobacterium lactis freeze-dried powder, 8.5kg of stachyose, and 31kg of glucose , whole milk powder 24kg, isomaltooligosaccharide 3.5kg, xylitol 2kg, resistant dextrin 2kg, banana powder 5.906kg, vitamin B 1 0.0015kg, Vitamin B 2 0.001kg, Vitamin B 6 0.001kg and zinc gluconate 0.0905kg.
[0054] 2. Preparation method
[0055] 1) first vitamin B 1 0.0015kg, Vitamin B 2 0.001kg, vitamin B 6 0.001kg and zinc gluconate 0.0905kg were mixed, and then mixed with g...
Embodiment 2
[0058] 1. Sensory evaluation
[0059] Get 6 grams of the product prepared in Example 1, add it to 30-40ml of warm water (below 40°C), and dissolve it completely. Choose 10 sensory assessors, after observing and tasting, carry out comprehensive evaluation to the color and luster, state, nourishment, smell of product of the present invention according to table 1 evaluation method, get average score as comprehensive score.
[0060] Table 1 Sensory evaluation methods and results
[0061]
[0062] The conclusion of the sensory evaluation is drawn from Table 1: the color of the product of the present invention is off-white to light yellow, uniform, powdery, no agglomeration, no foreign impurities visible to the naked eye, and the mouthfeel is fragrant and sweet, with a pleasant taste and no peculiar smell.
[0063] 2. Immunity comparison experiment
[0064] Experimental animals: Kunming mice aged 4-5 weeks, 18-22 g, male, 10 in each group, 90 in total.
[0065] Experimental co...
Embodiment 3
[0086] Lactobacillus reuteri lyoprotectant screening:
[0087] The protective agent generally has more than three hydrogen bonds or some ionic groups, which can replace water and combine with the protein of the bacteria during the protection process, thereby reducing the formation of ice crystals and protecting the bacteria from damage. The sterilization conditions of the protective agent are temperature 115°C and sterilization time 30min.
[0088] As a protective agent, skimmed milk powder can form a protective layer on the cell surface to protect the cell wall from damage. So choose skim milk powder as the basic protective agent. Glucose and sucrose were added to the basic protective agent at a ratio of 2%, 4%, 6%, 8%, and 10%, respectively, and the effects of adding different concentrations of glucose and sucrose on the protective effect of Lactobacillus reuteri were investigated.
[0089] Table 4 The impact of glucose and sucrose on the survival rate of Lactobacillus reu...
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