Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method of preserving Caenorhabditis elegans

A technology of Caenorhabditis elegans and Escherichia coli, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of death, inability to provide food and energy for nematodes, and achieve long recovery time and low survival rate. Effect

Inactive Publication Date: 2021-04-30
HARBIN INST OF TECH AT WEIHAI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the factors that limit the above-mentioned storage time to only 2-3 months is that E.coli OP50 strains will continue to grow at 4°C, gradually exhaust all nutrients, and then begin to die and lyse themselves, making it impossible to further Nematodes provide suitable food and energy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method of preserving Caenorhabditis elegans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0026] (4) Selection of Caenorhabditis elegans for preservation in embodiment 4

[0027] Select a petri dish with more L1 and L2 stage Caenorhabditis elegans under starvation state, and pick more than a dozen L1 and L2 stage Caenorhabditis elegans and place them in the gap of NGM solid medium that has been added with LB liquid medium. Carefully seal the Petri dish with parafilm and store it in a 4°C refrigerator for long-term storage.

[0028] (5) Embodiment 5 maintains culture system

[0029] In population ecology, the highest environmental capacity (referred to as "environmental capacity", K value) refers to the maximum number of populations that can be tolerated by a specific environment. The solid / liquid mixed medium ecological system constructed by the method of the present invention can ensure that the reproduction of E.coli OP50 in the S2 area is basically not disturbed by Caenorhabditis elegans and is more suitable for the growth of Caenorhabditis elegans, and it can ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biotechnology, and relates to a simple and effective long-term preservation method of Caenorhabditis elegans used in experiments. This method has the disadvantages of the traditional method for preservation experiments with Caenorhabditis elegans, and adopts a solid / liquid mixed medium to cultivate Caenorhabditis elegans to achieve the purpose of long-term (5-7 months) preservation of Caenorhabditis elegans. The outstanding advantages of the present invention are simple preservation operation, simple experimental method, short nematode recovery time and high survival rate.

Description

technical field [0001] The present invention relates to a method for preserving experimental Caenorhabditis elegans for experiments in the field of biotechnology, especially the -80°C preservation condition in the traditional preservation method cannot be provided in the experimental site, and the activity of nematodes preserved at a general low temperature of 4-15°C is often When it is greatly reduced, it provides a new possibility for long-term preservation of nematodes. Background technique [0002] Caenorhabditis elegans, belonging to the phylum Nematodes and the class Nematodes, is a non-parasitic nematode with a transparent body and a length of about 1 mm. It is mainly distributed in the soil in temperate regions. The number of cells is small, the function is not complicated, and the body is transparent. It is an ideal model animal for studying biology. Caenorhabditis elegans is widely used in the research of cell development and differentiation, nervous system, aging...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01N1/02
CPCA01K67/033A01N1/0226
Inventor 王虹元张艾涵谷庆泽王晋张治洲
Owner HARBIN INST OF TECH AT WEIHAI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com