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Biomarker composition for predicting or diagnosing postoperative recurrence of brain glioblastoma as well as application and kit thereof

A brain malignant glioma and biomarker technology, which is applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., to achieve the effect of simple and easy method and high accuracy.

Active Publication Date: 2018-11-06
BEIJING NEUROSURGICAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in view of the complexity of marker selection and combination, there are no detection methods and special detection kits for predicting distant invasion and / or metastasis of recurrent brain malignant glioma. Such marker panels and kits

Method used

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  • Biomarker composition for predicting or diagnosing postoperative recurrence of brain glioblastoma as well as application and kit thereof
  • Biomarker composition for predicting or diagnosing postoperative recurrence of brain glioblastoma as well as application and kit thereof
  • Biomarker composition for predicting or diagnosing postoperative recurrence of brain glioblastoma as well as application and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] This embodiment is a kit for predicting postoperative recurrence of glioma.

[0102] Above-mentioned kit comprises qRT-PCR amplification primer, and this amplification primer comprises:

[0103] The upstream primer sequence of exosome EGFRvIII is shown in SEQ ID NO:1 in the sequence listing, and the downstream primer sequence is shown in SEQ ID NO:2 in the sequence listing; and / or

[0104] The upstream primer sequence of exosome VEGF-A is shown in SEQ ID NO:3 in the sequence listing, and the downstream primer sequence is shown in SEQ ID NO:4 in the sequence listing; and

[0105] The upstream primer sequence of exosome VEGF165 is shown in SEQ ID NO:5 in the sequence listing, and the downstream primer sequence is shown in SEQ ID NO:6 in the sequence listing; and

[0106] The upstream primer sequence of exosome VEGF165b is shown in SEQ ID NO:7 in the sequence listing, and the downstream primer sequence is shown in SEQ ID NO:8 in the sequence listing; and

[0107] The ups...

Embodiment 2

[0114] This embodiment is a method for predicting postoperative recurrence of glioma.

[0115] The above method comprises the following steps:

[0116] 1. Extract exosomes

[0117] Proceed as follows:

[0118] (1) Collect fresh cerebrospinal fluid of patients in a centrifuge tube, centrifuge at 300g for 15 minutes at 4°C to remove suspended cells and cell debris in the medium;

[0119] (2) Transfer the supernatant into a new centrifuge tube, centrifuge at 10,000g for 30 minutes at 4°C to remove organelles and other impurities;

[0120] (3) Transfer the supernatant into a 30KD concentrator tube, concentrate by centrifugation at 3000g at 4°C, and concentrate the medium to 1 / 30 to 1 / 50 of the original volume;

[0121] (4) Mix the concentrated medium with Total Exosome Isolation (Thermo, 4478359) at a volume ratio of 2:1, shake and mix, and incubate overnight at 4°C;

[0122] (5) Then the incubated mixture was centrifuged at 4°C and 10,000g for 1 hour, and the precipitate was ...

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Abstract

The invention discloses biomarker composition for predicting or diagnosing postoperative recurrence of brain glioblastoma as well as application and a kit thereof. The biomarker exists in exosome of cerebrospinal fluid. The biomarker composition is prepared from VEGF165, VEGF165b and miR-21; the biomarker composition further is prepared from at least one of miR-10b, miR-485-5p, EGFRvIII and VEGF-A. The biomarker composition can predict and diagnose recurrence metastasis and in-situ recurrence of the brain glioblastoma in the ventricle and at the far position of the meninx and the accuracy is high.

Description

technical field [0001] The invention belongs to the field of diagnosis and prediction of brain glioma recurrence, in particular to a combination of biomarkers for predicting or diagnosing postoperative recurrence of brain malignant glioma, its application and a kit, which can predict brain malignant glioma in the ventricles, Distant recurrence and metastasis such as meninges. Background technique [0002] Glioma is the most common tumor of the central nervous system, accounting for about 45% of intracranial tumors. Histology is divided into four grades: grade I and grade II are low malignancy and have benign biological characteristics. After surgery, radiotherapy and After comprehensive treatment such as chemotherapy, the 5-year survival rate is 45-60%. Grades III and IV are high-grade gliomas, especially glioblastomas. With chemotherapy and other comprehensive treatment measures, the median survival time is only 18 months (III) and 12 months (IV). [0003] In recent years...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 刘福生张俊文苏晓东张梦梦金贵善米蕊芳
Owner BEIJING NEUROSURGICAL INST
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