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A kind of pure white black fungus strain and its application

A black fungus, white technology, applied in the field of pure white black fungus strain JAUH-W-591, can solve the problems of low foaming rate, long back hair, hard texture, etc., achieve high foaming rate, improve texture, water absorption Good results

Active Publication Date: 2021-07-16
泌阳县玉润农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the jade fungus still cannot change the following defects: (1) The texture is hard and brittle, without the texture of black fungus, and the taste is not as good as that of black fungus; (2) The back hair is long and not smooth enough
(3) The foaming rate is low, the foaming rate is only about 1:9
(4) Not resistant to low temperature

Method used

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  • A kind of pure white black fungus strain and its application
  • A kind of pure white black fungus strain and its application
  • A kind of pure white black fungus strain and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1 pure white black fungus JAUH-W-591 cultivation method of the present invention

[0045] (1) Production of mother species, the mother species medium of pure white black fungus JAUH-W-591 is PDA medium: 200g of peeled potatoes, 20g of glucose, 20g of agar, 1000mL of water, pH=6.5~6.7, the peeled Potato cut 1cm 3 See square pieces, boil in boiling water for 15-20min, filter with 6 layers of gauze, boil the filtrate again, add agar and keep stirring, add glucose after the agar is completely dissolved, set the volume to 1000mL, put it into a test tube or Erlenmeyer flask, Rubber stoppers or cotton plugs are sealed and covered with aluminum foil, sterilized at 121°C for 30 minutes under high pressure, cooled to about 25°C, inserted into the ultra-clean workbench with JAUH-W-591 strains, and then placed in a 25°C constant temperature incubator. Cultivate in dark light for about 12 days, and the mother species can grow well;

[0046] (2) Pure white black fungus J...

experiment example 1

[0054] Experimental Example 1 Separation and purification of mutant strain "JAUH-W-01"

[0055] The separation and purification process of the white black fungus mutant strain "JAUH-W-01" of the present invention comprises the following steps:

[0056] 1) Natural drying white black fungus mutant strain, the specimens are preserved in the Engineering Research Center of Edible Fungus Ministry of Education, Jilin Agricultural University;

[0057] 2) Preparation of culture medium

[0058] PDA medium: 200g peeled potato, 20g glucose, 20g agar, 1000ml water, pH=6.5~6.7, cut the peeled potato into 1cm 3 See square pieces, boil in boiling water for 15-20min, then filter with 6 layers of gauze, boil the filtrate again, add agar and keep stirring, add glucose after the agar is completely dissolved, and dilute to 1000ml. Put it into a test tube or a Erlenmeyer flask, seal it with a rubber stopper or cotton stopper and cover it with aluminum foil. Sterilize under high pressure at 121°C...

experiment example 2

[0061] Experimental example 2 Isolation and purification of white black fungus strain "JAUH-W-02"

[0062] 1) Preparation of liquid culture medium

[0063] Put 10g of peptone, 10g of glucose, 5g of sodium chloride, and 5g of beef extract into a large beaker; add 1000ml of water, and adjust the pH to 6.8-7.5 after it is completely dissolved; divide it into a small triangle bottle (200ml), and plug the bottle Plug the kraft paper and tie it; the culture medium is sterilized and kept at 121°C for 15 minutes;

[0064] 2) Production of liquid strains

[0065] Uncover the plate cover with the uncovering clip, and clamp the plate with the clip supporting the plate; scrape off the aerial hyphae of JAUH-W-01 on the surface of the plate with an inoculation hook; use an inoculation knife to cut evenly at a distance of 2cm from the old inoculation block 3-5mm size JAUH-W-01 bacteria block; fix the plate in the clip of the plate, put it on the tripod, burn the inoculation hook, put it on...

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Abstract

The invention relates to a pure white black fungus strain and its application, belonging to the technical field of edible fungi. Pure white black fungus strain JAUH‑W‑591, Auricularia heimuer, the preservation number in the General Microbiology Center of China Microbiological Culture Collection Management Committee is: CGMCC No.15374, the preservation date is April 11, 2018, and the address is: Chaoyang District, Beijing No. 3, No. 1 Yard, Beichen West Road, Institute of Microbiology, Chinese Academy of Sciences. The advantages are: pure white black fungus JAUH‑W‑591 has a color as white as jade, smooth and translucent, good water absorption, and a high foaming rate, which can reach about 1:15. After soaking, the texture is tender, slippery, sticky and waxy, and the taste is good , can be cultivated overwintering in Northeast China. While changing the original single color of the black fungus, it also effectively improves the product properties such as texture and mouthfeel of the black fungus, making the product unique and competitive in the market.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a pure white black fungus strain JAUH-W-591 and its application. Background technique [0002] Black fungus (Latin name Auriculariaheimuer) belongs to Basidiomycota, Agaricomycetes, Auriculares, Auriculariaceae, and Auricularia. Also known as light fungus, black ear, cloud ear, black cabbage and so on. China is the first country in the world to cultivate fungus. The earliest record about the method of planting fungus is "boil the porridge and put it on the trees, and cover it with grass, that is, the fungus." mentioned. The artificial cultivation of black fungus originated in China around 600 AD, and is the first artificially cultivated edible fungus species in the world. In 2017, the total output of black fungus in my country reached more than 6 million tons, making it the fourth largest cultivated edible fungus in the world. [0003] Black fungus is rich in nutrition a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/00C12Q1/6895C12N15/11A01H15/00A23L31/00A23L33/00
CPCA01H15/00A23L31/00A23L33/00A01G18/00C12Q1/6895C12N1/145C12R2001/645
Inventor 李晓
Owner 泌阳县玉润农业科技有限公司
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