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Method for curing sandy soil by using renewable calcium resource through MICP(microbially induced calcite precipitation)

A technology of microorganisms and calcium carbonate, which is applied in the field of building materials, can solve the problems of concrete performance degradation and high price, and achieve the effects of cost reduction, good strength and shape, and good economic and environmental protection

Inactive Publication Date: 2018-11-02
GUANGDONG UNIV OF TECH
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  • Abstract
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Problems solved by technology

Calcium chloride is used as the calcium source in most experimental studies, but its price is relatively expensive, and chloride is corrosive to the steel bars in the concrete structure, which will cause the degradation of concrete properties

Method used

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  • Method for curing sandy soil by using renewable calcium resource through MICP(microbially induced calcite precipitation)
  • Method for curing sandy soil by using renewable calcium resource through MICP(microbially induced calcite precipitation)
  • Method for curing sandy soil by using renewable calcium resource through MICP(microbially induced calcite precipitation)

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Embodiment 1

[0034] Such as Figure 6 Shown, the inventive method comprises the steps:

[0035] 1. Preparation of culture medium

[0036] Select 20±0.3g / L yeast extract, 10±0.2g / L ammonium sulfate, and 2±0.03g / L sodium hydroxide to mix to obtain the culture medium, and adjust the pH value of the culture medium to 9±0.1. A good nutrient solution is sterilized by high temperature and then sterilized by ultraviolet light. The high temperature sterilization temperature is 121±1°C, and the sterilization time is 20±5min.

[0037] 2. Preparation of bacterial solution

[0038] After the Bacillus sarcina is activated on the plate, the culture solution obtained in step S1 is inoculated with strains, and after the inoculation of the strains is completed, it is placed in a water bath constant temperature oscillation box to oscillate, the temperature is set at 27-30°C, and the rotation speed is 160-180rpm. After continuous cultivation for 16-19 hours, if white turbidity occurs, the culture of inocu...

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Abstract

The invention provides a method for curing sandy soil by using renewable calcium resource through MICP(microbially induced calcite precipitation). The method comprises the following steps: S1, preparation of a nutrient solution; S2, preparation of a bacteria solution; S3, preparation of a renewable calcium resource nutrient solution; and S4, preparation of biological sand pulp, wherein fresh oyster shells, scallop shells and egg shell from kitchen residuals are used as a renewable calcium resource, according to the method provided by the invention, the renewable calcium resource is subject toacid pickling so as to obtain calcium ions which are applied to the sandy soil application technology, kitchen wastes are used, solidifying and curing to the sandy soil are further performed, and materials obtained are excellent in intensity and appearance. Compared with the prior art that calcium is adopted for chemical analysis, the method provided by the invention has the advantages that the cost is greatly reduced, and the economical efficiency and the environmental protection effect are relatively good.

Description

technical field [0001] The invention belongs to the technical field of building materials, and more specifically relates to a method for using a regenerated calcium source to induce calcium carbonate precipitation and solidify sandy soil through microorganisms. Background technique [0002] Microbe-induced calcium carbonate precipitation and solidification of sandy soil technology is an emerging environmental-friendly soil improvement technology. This technology mainly generates carbonate ions by hydrolyzing urea through the urease metabolized by microorganisms themselves, and combines with surrounding calcium ions to form calcium carbonate precipitation and cement sandy soil. Particles can improve the mechanical properties of soil. [0003] The technology of microorganism-induced calcium carbonate precipitation and stabilization of sandy soil needs to consume a large amount of calcium ions. Calcium chloride is selected as the calcium source in most experimental studies, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01B79/02E02D3/12C12N1/20C05G1/00C12R1/01
CPCA01B79/02C05C9/00C12N1/20E02D3/12C05F1/005C05F1/007
Inventor 牛九格梁仕华龚星冯德銮罗庆姿
Owner GUANGDONG UNIV OF TECH
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