Method, cultivation method and application of removing endophytic bacteria in sagittarius cultivating process

A cultivation method and technology of Sagittarius, which is applied in the field of treatment of proliferating Sagittarius tissue culture seedlings, and can solve the problems of insufficient supply of culture materials and high endophyte contamination rate

Active Publication Date: 2021-09-10
FUJIAN AGRI & FORESTRY UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This patent document only discloses a method of how to quickly propagate Arthroatia tissue cultured seedlings. In this method, detoxification is carried out before solid culture, and the solid culture is first put into intermittent immersion liquid culture. The endophyte contamination rate of solid culture in the early stage is relatively low High, unable to supply sufficient culture materials, did not deal with the endophytes produced during the culture process

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  • Method, cultivation method and application of removing endophytic bacteria in sagittarius cultivating process
  • Method, cultivation method and application of removing endophytic bacteria in sagittarius cultivating process

Examples

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Embodiment 1

[0035] Embodiment 1: the impact of streptomycin sulfate on Sagittarius tissue cultured seedlings

[0036] In this embodiment, the Arthroatia sagitta tissue culture seedlings are cultivated using a semi-solid medium, wherein the formula of the semi-solid medium includes: MS+2.5mg / L of 6-BA+0.01mg / L of NAA+3.5g / L of carrageenan +30g / L sucrose, each bottle contains 30ml medium.

[0037] As can be seen from the following table 1, when the concentration of streptomycin sulfate was 50mg / L, the pollution rate of arrowhead tissue cultured seedlings was 42%, the subculture multiplication factor was 1.0, and the rooting rate was 85%; When the concentration of streptomycin was 200mg / L, the pollution rate of sagittarius tissue cultured seedlings was reduced to 23%, the subculture multiplication factor was 2.1, and the rooting rate was 72%; however, when the concentration of streptomycin sulfate was 400mg / L, sagittarius The pollution rate of the tissue culture seedlings was almost the sam...

Embodiment 2

[0041] Embodiment 2: the influence of ampicillin on the tissue culture seedling of sagittarius

[0042] In this example, the tissue-cultured seedlings of Sagittarius quinceus were cultured using a semi-solid medium, wherein the formula and volume of the semi-solid medium were the same as those in Example 1.

[0043] As can be seen from the following table 2, when the concentration of ampicillin was 50mg / L, the pollution rate of sagittarius tissue cultured seedlings was 62%, the subculture multiplication factor was 1.0, and the rooting rate was 73%; when the concentration of ampicillin was 150mg / L, the pollution rate of Sagittarius tissue-cultured seedlings was 42%, the subculture multiplier was 2.0, and the rooting rate was 80%; and when the concentration of ampicillin was 200mg / L, the pollution rate of Sagittarius tissue-cultured seedlings was reduced to 35%. %, the subculture multiplier no longer increased, and was still 2.0, and the rooting rate began to decrease, which wa...

Embodiment 3

[0047] Embodiment 3: the impact of streptomycin sulfate and ampicillin on Sagittarius tissue culture seedlings in semi-solid culture

[0048] In this example, the tissue-cultured seedlings of Sagittarius quinceus were cultured using a semi-solid medium, wherein the formula and volume of the semi-solid medium were the same as those in Example 1.

[0049] Streptomycin sulfate and ampicillin are made into a solution, wherein the preparation method of the streptomycin sulfate solution comprises: weighing 50 mg of powdery streptomycin sulfate, dissolving it in absolute ethanol, and finally settling to 1 L to prepare Concentration is the streptomycin sulfate solution of 50mg / L; The preparation method of described ampicillin solution comprises: take by weighing the powdery ampicillin of 50mg, be dissolved in water, be finally settled to 1L, make concentration be 50mg / L Ampicillin solution.

[0050] As can be seen from the following table 3, when the concentration of streptomycin sul...

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Abstract

The invention discloses a method for removing endophytic bacteria in the cultivation process of sagittarius, a cultivation method and application. The method for removing endophytes in the culturing process of Sagittarius comprises: adopting combined treatment of streptomycin sulfate and ampicillin, combining semi-solid medium and liquid medium for culturing, and removing endophytes in the process of culturing Sagittarius tissues. The method has high-efficiency bactericidal and antibacterial effects, eliminates the endophytic bacteria in the tissue culture process of sagittarius, and avoids the toxic and side effects of high-concentration antibiotics on the sagittarius; The slow growth and non-proliferation effect can reduce the endophytic bacteria contamination rate of Sagittarius tissue cultured seedlings to 10%, the subculture multiplier is about 3.5, and the rooting rate reaches 90%, which can ensure the normal growth of Sagittarius tissue cultured seedlings; the method Used in the treatment of contaminated tissue culture seedlings in the multiplication state, it can completely eliminate the endophytes in the process of subculture proliferation and rooting of Sagittarius tissue culture, and successfully solve the bottleneck problem that endophytes are difficult to remove in the production process of Sagittarius tissue culture .

Description

technical field [0001] The invention belongs to the technical field of sagittarius cultivation, and in particular relates to a method for removing endophytic bacteria in the cultivation process of sagittarius, a cultivation method of sagittarius and a treatment method for proliferating sagittarius tissue cultured seedlings. Background technique [0002] The seeds and seedlings in the Sagittarius production area are all kept from traditional bulbs, and the varieties are degrading day by day. The performance is that the yield is decreasing year by year, the quality is poor, and the occurrence of serious pests and diseases, especially the occurrence of smut and leaf spot, has caused a large area of ​​production reduction. The tissue-cultured seedlings grow neatly and robustly, are easy to manage, and are easy to obtain high-quality and high-yield bulbs, which can improve the commerciality of bulbs, and can effectively solve the problems of provenance with diseases and variety de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高美萍林志城何新华罗聪熊发前
Owner FUJIAN AGRI & FORESTRY UNIV
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