Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Self-assembled nanostructures and separation membranes comprising aquaporin water channels and methods of making and using them

A technology of aquaporin and nanostructure, which is applied in the field of self-assembled nanostructures, and can solve problems such as difficulty in controlling the size of vesicles

Active Publication Date: 2018-10-23
AQUAPORIN AS
View PDF15 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In practice, however, it is often difficult to control the final vesicle size, resulting in the formation of dispersed emulsions with vesicle diameters ranging from about 60 to 80 nm to about 1000 nm or more

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Self-assembled nanostructures and separation membranes comprising aquaporin water channels and methods of making and using them
  • Self-assembled nanostructures and separation membranes comprising aquaporin water channels and methods of making and using them
  • Self-assembled nanostructures and separation membranes comprising aquaporin water channels and methods of making and using them

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Preparation of Example 1.PEI-Aquaporin-Z Nanoparticles and Stopped Flow Testing (Stopped FlowTesting)

[0101] m W Poly(ethyleneimine) (linear) (PEI) of 4000 Da was purchased from Sigma Aldrich and used without any other purification. By adding 8g NaCl, 0.2g KCl, 1.44g Na 2 HPO 4 and 0.24g of KH 2 PO 4 Dissolve in 800 mL MilliQ purified HO 2 A 10 mM phosphate solution (PBS) (pH 7.2, 136 mM NaCl, 2.6 mM KCl) was prepared by adjusting the pH to 7.2 with HCl in 0 and bringing the volume up to 1 L. N,N-Dimethyldodecylamine N-oxide BioXtra (lauryldimethylamine N-oxide) (99% purity), LDAO was purchased from Sigma Aldrich.

[0102] The PEI-based self-assembled structures were prepared by a direct dissolution method. First, 100 ml of 5 mg / mL PEI in phosphate buffer at pH 7.2 was prepared by dissolving 500 mg of PEI powder in 100 mL of PBS at pH 7.2. Further, 100 mL of 5 mg / mL PEI was directly mixed with 1.5 mg of aquaporin Z (AQPZ) (0.3 mL from a purified stock solution...

Embodiment 2

[0115] Example 2. Preparation and testing of PEI nanostructures with AQP9 and AQP12

[0116] In a similar manner, PEI-based self-assembled structures containing human aquaporins, more specifically aquaporin-9 and aquaporin-012, were prepared by the direct dissolution method. First, 100 ml of 5 mg / mL PEI in phosphate buffer at pH 7.2 was prepared by dissolving 500 mg of PEI powder in 100 mL of PBS at pH 7.2. Further, 100 mL of 5 mg / mL PEI was mixed with 1.5 mg aquaporin-9 (0.15 mL from a purified stock solution of 10 mg / mL aquaporin-9 in 2% LDAO) and aquaporin-12 (from 9 mg / mL mL of aquaporin-12 was directly mixed with 0.17 mL of the purified stock solution in 2% LDAO, corresponding to a two-component mixture at a molar ratio of 1 / 800 AQP / PEI. The PEI-AQP mixture was stirred overnight at 170 rpm for no more than 20 hours (but not less than 12 hours).

[0117] After stirring, the mixture was transferred to a 100 mL glass bottle the next day, kept at room temperature, and measu...

Embodiment 3

[0122] Example 3. Fabrication and testing of nanostructures with PEI 800b and 1000b

[0123] In a similar manner, PEI 4000 Da self-assembled structures containing AQPZ, and 800-branched and 1000-branched PEI were prepared by the direct solution method. First, prepare 100 ml of 5 mg / mL phosphate salts of 800-branched and 1000-branched PEI at pH 7.2 by dissolving 500 mg of 800-branched and 1000-branched PEI powder in 100 mL of PBS at pH 7.2 buffer. Further, 100 mL of 5 mg / mL PEI was directly mixed with 1.5 mg of AqpZ (0.3 mL from a purified stock solution of 5 mg / mL AqpZ in 2% LDAO), corresponding to a two-component mixture at a molar ratio of 1 / 800 AQP / PEI . The PEIAQP mixture was stirred overnight at 170 rpm for no more than 20 hours (but not less than 12 hours).

[0124] After stirring, the mixture was transferred to a 100 mL glass bottle the next day, kept at room temperature, and measured the next day. The size and permeability of the PEI-AQPZ self-assembled structures ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to self-assembled nanostructures comprising polyalkyleneimine (PAI) and a detergent solubilized transmembrane protein, such as an aquaporin protein.

Description

technical field [0001] The present invention relates to self-assembled nanostructures formed between transmembrane proteins, such as aquaporin water channel (AQP) and polyalkyleneimine (polyalkyleneimine, PAI), and filtration devices comprising said nanostructures membrane. The invention also relates to nanostructures and separation membranes, such as hollow fibers and processes for the preparation of hollow fiber modules, and uses thereof. Background technique [0002] The use of amphiphilic lipids and block copolymers to form self-assembled vesicles with bilayer or bilayer-like structures is well known in the art, especially for the immobilization of amphiphilic membrane proteins such as aquaporin water channels (AQP). The AQP-containing vesicles can then be used to prepare membranes with immobilized AQP for applications such as water purification (WO2006 / 122566) or generation of salinity power (WO2007 / 033675), usually by Vesicles are deposited as a layer or thin film o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01D61/02B01D63/02B01D69/08B01D69/12B01D69/14B01D71/56
CPCB01D61/002B01D61/025B01D69/125B01D69/144B01D71/56B01D69/08Y02A20/131B82Y30/00B82Y40/00
Inventor 玛丽安娜·施普尔伯克日什托夫·恰斯库斯
Owner AQUAPORIN AS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com