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Positive relationship of AQP1 expression and beta-catenin expression

A relational, positive technology, applied in the field of biomedicine

Inactive Publication Date: 2016-12-28
宁波美丽人生医药生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant clinical data to confirm the relationship between AQP1 and β-catenin in human tumor tissues

Method used

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  • Positive relationship of AQP1 expression and beta-catenin expression
  • Positive relationship of AQP1 expression and beta-catenin expression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Overexpression of AQP1 improves the proliferation and invasion of LN229 cells

[0056] Previous studies have shown that AQP1 can enhance the cell migration ability of HT20, 12 / 40B16F10 and 4T1 cell lines (Jiang 2009, Hu&Verkman 2006). In order to study the role of AQP1 in glioma, we used Western Blot method to detect the expression of AQP1 in homologous LN229 cell line. AQP1 expression was not detected in LN229 cells ( figure 1 A). Then, we transfected the plasmid expressing the full-length AQP1 sequence into LN229 cells to obtain LN229 cells stably expressing AQP1, and named it AQP1 / LN229 cell line. The empty vector was transfected into LN229 cells to obtain LN229 cells stably expressing the empty vector, which were named vector / LN229 cell line as a control cell line. Compared with vehicle / LN229 cells, AQP1 protein expression level was significantly upregulated in AQP1 / LN229 cells ( figure 1 B). In order to complete the cellular localization of AQP1, an immunofluo...

Embodiment 2

[0060] Expression of AQP1 in human glioma tissues

[0061]In order to further explore the functional role of AQP1 in glioma tissues, based on the gene expression analysis data from the Rembrandt database, we further detected the expression level of AQP1 mRNA. (Molecular Brain Tumor Data Lookup, https: / / caintegrator.nci.nih.gov / rembrandt / ). These data included glioma tissues (n=454) as well as non-tumor tissues (n=24). Valid data showed that AQP1mRNA levels were upregulated in glioma tissues (1.96±0.29) compared with non-tumor tissues ( image 3 A). Secondly, we used Western Blot to detect the expression level of AQP1 protein in various WHO grade gliomas ( image 3 B). Two normal brain samples and one nerve sheath sample were used as controls. It was found by Western Blot that the expression of AQP1 was not detected in the lysate of non-tumor tissue and schwannoma samples. In contrast, AQP1 expression levels were upregulated in human glioma tissues. Furthermore, AQP1 was...

Embodiment 3

[0063] Expression of AQP1 in patients with glioma

[0064] Interestingly, we observed that AQP1-immunohistochemistry showed specific patterns ( Figure 4 Aa-j). In contrast to previous studies, AQP1 expression was not detected in glial cells of normal brain tissue (Saadoun et al. 2002a, Hayashi et al. 2007). In contrast, AQP1 expression levels were upregulated in tumor regions ( Figure 4 Aa). In astrocytoma ( Figure 4 Ab) and oligodendroglioma ( Figure 4 Ac) AQP1 expression was observed. In astrocytomas, AQP1 staining presents a typical astrocyte appearance. In low-grade glioma endothelial cells, AQP1 expression was observed in human glioma tissue sections, whereas AQP1 expression was absent in proliferating endothelial cells of high-grade glioma. In addition, AQP1 immune responses were more aggregated and perivascularly distributed ( Figure 4 Af). Further observations revealed that AQP1 was expressed in tumor-infiltrated areas ( Figure 4 Ag) was expressed and l...

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Abstract

The invention relates to the field of biological medicines and especially relates to a positive relationship of aquaporin AQP1 expression and a beta-catenin expression level. The inventor explores expression of AQP1 in a specific cell model and a clinical sample and an action form of AQP1 overexpression-caused beta-catenin up-regulated expression in a glioma patient. Through use of the specific cell model and a clinical sample in exploration of beta-catenin expression, a relationship of AQP1, beta-catenin and glioma patient prognosis and a relationship of AQP1 and a beta-catenin signaling mechanism are ensured and the positive relationship of aquaporin AQP1 expression and a beta-catenin expression level is further confirmed.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the positive relationship between AQP1 expression and β-catenin expression. Background technique [0002] Aquaporins (AQPs) belong to a family of small hydrophobic intrinsic membrane proteins that mediate the transport of water molecules across membranes involving osmotic pressure gradients (Verkman 2005). There are 13 aquaporins (AQP-AQP12) in mammals. Such AQPs can be divided into two functional groups: traditional aquaporins (AQP 1, 2, 4, 5, 8), which only facilitate the transport of water molecules; glycerol aquaporins (AQP3, 7, 9, 10), which also Transport of small uncharged molecules such as glycerol, lactic acid and urea (verkman 2005). [0003] Because of the close relationship between AQPs and tumor development, its research has attracted public attention. AQP3 expression was upregulated in liver, colon, and cervical cancers, as well as in esophageal squamous cell carcinoma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/68G01N33/57484G01N2800/7028
Inventor 田晓丽
Owner 宁波美丽人生医药生物科技发展有限公司
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