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Water-soluble yeast beta-glucan as well as preparation method and application thereof

A glucan and water-soluble technology, applied in the field of water-soluble yeast beta-glucan and its preparation, can solve the problems of unsatisfactory water solubility of products and high equipment requirements

Pending Publication Date: 2018-10-02
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires high equipment, and the water solubility of the product is not ideal

Method used

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  • Water-soluble yeast beta-glucan as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Add 20kg of water-insoluble yeast β-glucan to prepare 1000L solution with purified water, adjust pH to 4.0, temperature 20°C, add glucanase to make the concentration reach 0.1U / mL, keep warm for 24h, and collect the supernatant by centrifugation. The supernatant was filtered with 100 mesh diatomaceous earth. Collect the supernatant, adjust the pH value to 7, use an organic membrane with a molecular weight cut-off of 10KD for membrane separation, collect the retentate, and obtain a total of 400L of retentate. The retentate presents a clear solution, which is water-soluble β-glucan.

[0045] The retentate was added to freeze-dry to obtain 12.1 kg of powdered water-soluble yeast β-glucan product (ie, the amount of dry matter accounted for 3.1% of the content of the retentate). The weight average molecular weight of the product is 158KD, the content of β-glucan is 85.8%, and the product yield is 60%.

[0046] Wherein, the amount of dry matter is the amount of the powdered ...

Embodiment 2

[0051] Add 100kg of water-insoluble yeast β-glucan to 1000L of purified water to prepare a solution of 1000L, adjust the pH to 7.0, the temperature is 70°C, add glucanase to make the concentration reach 5U / mL, incubate for 1 hour, and collect the supernatant by centrifugation. The supernatant was filtered with 200 mesh diatomaceous earth. Collect the supernatant, adjust the pH value to 9, use an organic membrane with a molecular weight cut-off of 10KD for membrane separation, collect the retentate, and obtain a total of 800L of retentate. The retentate presents a clear solution, which is water-soluble β-glucan.

[0052] The retentate was added to freeze-dry to obtain 56 kg of powdery water-soluble yeast β-glucan product (that is, the amount of dry matter accounted for 7% of the content of the retentate). The weight average molecular weight of the product is 366KD, the content of β-glucan is 82.1%, and the product yield is 56%.

[0053] Embodiment two adopts the same method as...

Embodiment 3

[0055] Add 50kg of water-insoluble yeast β-glucan to prepare 1000L of solution with purified water, adjust pH to 5.0, temperature at 50°C, add dextranase to make the concentration reach 1U / mL, incubate for 8h, and collect supernatant by centrifugation. The supernatant was filtered with 100 mesh diatomaceous earth. Collect the supernatant, adjust the pH value to 8, use an organic membrane with a molecular weight cut-off of 10KD for membrane separation, collect the retentate liquid, and obtain a total of 600L retentate liquid. The retentate liquid presents a clear solution, which is water-soluble β-glucan.

[0056] The retentate was added to freeze-dry to obtain 35 kg of powdery water-soluble yeast β-glucan product (that is, the amount of dry matter accounted for 5.83% of the content of the retentate). The weight average molecular weight of the product is 456KD, the content of β-glucan is 83.3%, and the product yield is 70%.

[0057] Embodiment 3 is determined by the same metho...

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Abstract

The invention relates to the field of food processing, in particular to a water-soluble yeast beta-glucan as well as a preparation method and application thereof. The method comprises the following steps: enzymatic treatment: adding glucanase to a raw material containing water-insoluble yeast beta-glucan for treatment, and then separating and collecting supernatant; (2) filtering treatment: filtering the supernatant; (3) membrane separation: conducting membrane separation on the supernatant, and collecting trapped fluid to obtain the water-soluble yeast beta-glucan, wherein the glucanase is derived from aspergilli. A water-soluble yeast beta-glucan product obtained by the method provided by the invention is colorless, odorless, completely water-soluble, and maintains an original molecularstructure of a yeast glucan.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a water-soluble yeast beta-glucan and its preparation method and application. Background technique [0002] Yeast β-glucan is a polysaccharide present in the cell wall of yeast, which has various physiological functions such as enhancing immunity and anti-radiation. Among them, most of the naturally occurring glucans are water-insoluble glucans, accounting for about 85%. At present, the yeast β-glucan products obtained through extraction and purification processes are water-insoluble glucans. Yeast glucan has been approved as a new food ingredient by many countries, but its poor water solubility has limited its application range. [0003] In order to improve the water solubility of yeast glucan, chemical and physical modification methods can be used to modify its structure, among which carboxymethylation, sulfation and phosphorylation solubilization modification are used more ofte...

Claims

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Application Information

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IPC IPC(8): C12P19/08A61K31/716A61P37/04A61P39/00
CPCA23V2002/00A61K31/716C12P19/08A23V2250/5034
Inventor 张海波俞学锋李知洪姚鹃张彦彭宁
Owner ANGEL NUTRITECH CO LTD
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