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Prebiotic-probiotic compound micro-ecological preparation and preparation method thereof

A technology of compound microecology and probiotics, applied in the field of microbial fermentation, can solve the problems of decreased immunity of animals, single bacterial species, drug resistance, etc., to enhance immunity, diverse bacterial species, and promote body digestion Effect

Inactive Publication Date: 2018-10-02
吉林师范大学博达学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the 1940s, in order to promote the rapid growth of animals, antibiotics were added to the feed. However, with the long-term and extensive use of antibiotics, there will be drug resistance, drug residues, and the decline of animal immunity.
[0005] At present, the main probiotic preparations in the domestic and foreign markets are Bacillus subtilis preparations and lactic acid bacteria preparations, most of which are prepared from a variety of single strains, and the composition of the strains is relatively simple

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] 1) Preparation of probiotic starter:

[0019] Preparation of kefir starter: configure 10% skim milk solution, after sterilizing at 85-95°C for 10-30 minutes, cool to 20-30°C, add kefir directly according to 0.1-0.5% of the volume of skim milk Formula starter, fermented and cultivated at 23-28°C for 14-18 hours, and stored at 4°C for later use.

[0020] Preparation of Bacillus subtilis: Streak culture the frozen Bacillus subtilis, isolate typical colonies and inoculate them in liquid culture medium for 18 hours with shaking at 37°C. Microscopic examination conforms to the standard form of Bacillus subtilis, and the growth state is good , inoculate 3-6% of the inoculum in liquid medium for activation, shake culture at 37°C for 18-24 hours, and store at 4°C for later use.

[0021] 2) Mixed fermentation:

[0022] Medium configuration: Add 10-20g of concentrated whey protein, 6-8g of glucose, 3-5g of yeast powder, 3-6g of calcium carbonate, 0.1-0.3g of magnesium sulfate, 0...

Embodiment 1

[0027] 1) Preparation of probiotic starter

[0028] Preparation of kefir starter: configure 10% skim milk solution, after sterilizing at 85°C for 30 minutes, cool to 20°C, add kefir direct-injection starter according to 0.1% of the volume of skim milk, at 28°C Fermented and cultured for 14 hours under conditions, and stored at 4°C for future use.

[0029] Preparation of Bacillus subtilis: Streak culture the frozen Bacillus subtilis, isolate typical colonies and inoculate them in liquid culture medium for 18 hours with shaking at 37°C. Microscopic examination conforms to the standard form of Bacillus subtilis, and the growth state is good , inoculate 6% of the inoculum in liquid medium for activation, culture with shaking at 37°C for 18 hours, and store at 4°C for future use.

[0030] 2) Mixed fermentation

[0031] Medium configuration: Add 10g of concentrated whey protein, 8g of glucose, 5g of yeast powder, 3g of calcium carbonate, 0.3g of magnesium sulfate, 0.01g of mangane...

Embodiment 2

[0035] 1) Preparation of probiotic starter

[0036] Preparation of kefir starter: configure 10% skim milk solution, after sterilizing at 90°C for 20 minutes, cool to 25°C, add kefir direct-throwing starter according to 0.3% of the volume of skim milk, at 23°C Fermented and cultured for 18 hours under conditions, and stored at 4°C for future use.

[0037]Preparation of Bacillus subtilis: Streak culture the frozen Bacillus subtilis, isolate typical colonies and inoculate them in liquid culture medium for 18 hours with shaking at 37°C. Microscopic examination conforms to the standard form of Bacillus subtilis, and the growth state is good , according to the inoculum size 3%, inoculated in liquid medium for activation, 37 ° C, shaking culture for 24 hours, stored at 4 ° C for later use.

[0038] 2) Mixed fermentation

[0039] Medium configuration: Add 20g of concentrated whey protein, 6g of glucose, 3g of yeast powder, 6g of calcium carbonate, 0.2g of magnesium sulfate, 0.02g of...

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Abstract

The invention discloses a prebiotic-probiotic compound micro-ecological preparation and a preparation method thereof. The preparation method of the prebiotic-probiotic compound micro-ecological preparation comprises the following main steps of selecting lactic acid bacteria, yeast and bacillus subtilis as bacterial strains of the prebiotic-probiotic compound micro-ecological preparation, wherein the lactic acid bacteria and the yeast are from Kefir grains; separately activating the Kefir grains and the bacillus subtilis, and mixing the activated Kefir grains and bacillus subtilis at a ratio of3 to 1; inoculating a culture medium with the mixed probiotic stains, wherein the volume of the mixed probiotic stains accounts for 3-10% of the volume of the fermenataion liquid, so as to be subjected to fermentation culture; adding prebiotics, adding skimmed milk powder, trehalose, monosodium glutamate and glycerine as protective agents, carrying out uniform mixing, and performing low-temperature spray-drying on the mixture so as to obtain the micro-ecological preparation. The prebiotic-probiotic compound micro-ecological preparation is characterized by containing the lactic acid bacteria,the yeast and the bacillus subtilis, so that, micro-ecological balance of the body can be jointly adjusted; moreover, the prebiotic-probiotic compound micro-ecological preparation also contains preparation substances, so that, body health can be better improved so as to enhance body immunity.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a prebiotic and probiotic composite microecological preparation and a preparation method thereof. Background technique [0002] In the 1940s, in order to promote the rapid growth of animals, antibiotics were added to the feed. However, with the long-term and extensive use of antibiotics, there will be drug resistance, drug residues, and the decline of animal immunity. Probiotics are widely used because they are green, safe, and have no drug residues, gradually replacing antibiotics. Compared with traditional feed additives, probiotic microecological preparations can effectively inhibit the growth and reproduction of pathogenic bacteria and pathogenic microorganisms in the digestive tract, and make probiotics colonize in the intestinal tract, adjust the intestinal balance of animals, and play a role in the prevention and treatment of animal diseases. certain effect. ...

Claims

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Application Information

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IPC IPC(8): A23L33/21A23L33/135C12N1/20C12N1/16C12R1/645C12R1/125
CPCA23L33/21A23L33/135A23V2002/00C12N1/16C12N1/20A23V2200/30A23V2200/324A23V2200/32
Inventor 田木王艳丹赵琳琳解慧郝鑫周晓梅柴振宇
Owner 吉林师范大学博达学院
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