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Double channel automatic observation method of plant chlorophyll fluorescence

A chlorophyll fluorescence and automatic observation technology, applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of limited types of spectrometers and high requirements for the operating environment of spectrometers, so as to avoid attenuation and spectral shift and ensure Uniqueness, the effect of avoiding offset

Active Publication Date: 2018-09-28
NANJING UNIV
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Problems solved by technology

[0006] However, the observation of fluorescence requires a spectrometer with sub-nanometer spectral resolution, and the types of spectrometers available on the market are limited and can only receive one light path. Two; In addition, the operating environment of the spectrometer is high. In order to reduce the interference of noise, the spectrometer needs to be placed in a constant temperature box

Method used

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  • Double channel automatic observation method of plant chlorophyll fluorescence
  • Double channel automatic observation method of plant chlorophyll fluorescence
  • Double channel automatic observation method of plant chlorophyll fluorescence

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Embodiment Construction

[0039] The present invention is further explained by example below:

[0040] According to the step (1) of the technical solution, a prism chamber is made and an observation system is installed. attached figure 2 to attach Figure 5 Prism chamber structure and size drawing drawn for independent design, attached Image 6 In order to assemble the actual picture of the inside of the chamber with the prism, when performing field spectrum observation, the inside of the chamber needs to be blackened except for the prism to prevent the reflection of the inner wall of the chamber from affecting the optical path. attached Figure 7 In order to install a complete external actual picture of the prism chamber, the upper and lower light inlets are equipped with cosine correctors, the left light outlet is connected to the optical fiber, the other end of the optical fiber is connected to the spectrometer, and the left side is the motor. When installing the prism chamber, ensure that the ...

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Abstract

The invention provides a method for accurately inverting canopy sunlight induction of chlorophyll fluorescence through plant canopy spectral data obtained by a spectral observation system based on a prism splitting optical path and belongs to the research field of plant remote sensing inversion parameter acquisition methods. The method comprises building a hyperspectral observation system using prisms to split and switch optical paths, automatically and continuously acquiring hyperspectral data, calculating solar incidence and canopy reflection radiance, calculating canopy reflectivity and inverting chlorophyll fluorescence. Through rotation of the prisms, the solar incident spectrum and the canopy reflection spectrum are alternately acquired so that the uniqueness of the optical path entering the spectrometer is guaranteed, the attenuation of the double optical paths or the multiple optical paths to lights and the offset between the spectral ends are avoided, the noise interference ofthe fluorescence inversion is reduced, the continuous and high quality plant canopy fluorescence data products are obtained and the accuracy of land primary productivity monitoring is improved.

Description

1. Technical field [0001] The invention relates to a method for calculating canopy sunlight-induced chlorophyll fluorescence using the vegetation canopy spectral data continuously acquired by an automatic observation system. Canopy reflection spectrum, a method for calculating sunlight-induced chlorophyll fluorescence of vegetation canopy, belongs to the research field of vegetation remote sensing inversion parameter acquisition method. 2. Background technology [0002] The total primary productivity of vegetation refers to the fixation of CO by plants through photosynthesis. 2 The capacity is the production capacity of the terrestrial ecosystem under natural conditions. The total primary productivity of vegetation is the largest carbon flux in the global carbon cycle. The total primary productivity of vegetation directly determines the subsequent carbon cycle elements of terrestrial ecosystems, and is also related to the ability of terrestrial ecosystems to support human s...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486G01N2021/6417G01N2021/635
Inventor 张乾张永光徐翔刘志刚单楠李季
Owner NANJING UNIV
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