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Preparation method and use method of malachite green solution

A technology of malachite green solution, applied in the field of preparation of malachite green solution, to achieve clear restaining, wide applicability, and improved work efficiency

Inactive Publication Date: 2018-09-28
江苏中济万泰生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no acid-fast staining solution in the domestic market for counterstaining with malachite green. After staining and comparison, counterstained with malachite green solution, the negative bacteria of acid-fast staining are blue-green, the background is clearer, and the advantages are obvious.

Method used

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Examples

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Effect test

Embodiment Construction

[0022] The preparation method of the malachite green solution of the present invention is described in further detail below in conjunction with the examples.

[0023] The specific implementation steps of the preparation method of malachite green solution in the present embodiment are as follows:

[0024] A. Weigh 6 g of malachite green (oxalate) and dissolve it in 100 ml of 95% ethanol.

[0025] B. Add 850ml of purified water and stir evenly.

[0026] C. Filter with ordinary filter paper.

[0027] D. Add 50ml of glycerin and stir evenly and fill it into a black or brown bottle for later use.

[0028] The steps of the acid-fast staining method are as follows:

[0029] A. The fixed smear is placed on the slide rack

[0030] B. Add phenol fuchsin solution for staining. The reagent completely covers the sample and stays for 5 minutes. Rinse with running tap water until no cloudy dye appears, and drain the remaining water on the sample.

[0031] C. Add hydrochloric acid ethano...

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Abstract

The invention relates to a preparation method and a use method of a malachite green solution. The preparation method comprises the following steps: firstly, weighing 6g of malachite green (oxalate) and dissolving into 100ml of 95 percent ethanol; then adding 850ml of purified water for uniformly stirring and filtering by using ordinary filter paper; finally, adding 50ml of glycerol for uniformly stirring and filling into a black or brown bottle for later use. After staining contrast, the malachite green solution subjected to counterstaining has the advantages that acid-fast staining gram-negative bacteria are bluish green, the background is clearer and the advantages are obvious, so that the working efficiency of operators is improved, and wider applicability is realized.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for preparing and using a malachite green solution. Background technique [0002] Acid-fast staining method acid-fast staining method was first created by Ehrlich (F. Ehrlich) in 1882 and improved by F. Ehrlich Ziehl (Ziehl) to create a bacterial staining method. The most representative ones are Ziehl-Neelsen staining and Ziehl-Gabbet staining for Mycobacterium tuberculosis. [0003] Zie-Nissl's acid-fast staining method is to make mycolic acid and phenol fuchsin firmly combine to form a complex under heating conditions, and it will not be decolorized when treated with hydrochloric acid alcohol. After counterstaining with alkaline methylene blue, mycobacteria are still red, while other bacteria and the substances in the background are blue. [0004] The cell wall of acid-fast bacteria is rich in wax and lipid, which leads to low dyeing efficiency. In the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 赵树铭林裕翔陈亚平吴明磊
Owner 江苏中济万泰生物医药有限公司
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