Method for immobilizing anaerobic iron ammonia oxidizing bacteria through magnetic chitosan gel beads

A technology of chitosan gel balls and anaerobic iron and ammonia, applied in the direction fixed on/in the organic carrier, can solve the problems of river hazards, improve the living environment, improve the capacity, and improve the treatment efficiency Effect

Inactive Publication Date: 2018-09-28
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the harm caused by the large amount of sewage discharge to the river is becoming more and more serious, and it has become an environmental problem that needs to be solved urgently.

Method used

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  • Method for immobilizing anaerobic iron ammonia oxidizing bacteria through magnetic chitosan gel beads
  • Method for immobilizing anaerobic iron ammonia oxidizing bacteria through magnetic chitosan gel beads
  • Method for immobilizing anaerobic iron ammonia oxidizing bacteria through magnetic chitosan gel beads

Examples

Experimental program
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Effect test

Embodiment 1

[0043] A kind of preparation method of magnetic chitosan gel ball, carries out according to the following steps:

[0044] Add 2g chitosan in 100mL 1% acetic acid solution, make 2% chitosan solution, ferric salt (FeCl 3 ·6H 2 O) and ferrous salt (FeCl 2 4H 2 O) add in the chitosan solution according to the ratio of molar ratio 2:1, stir and make it mix uniformly, obtain Fe-containing 3 o 4 A monodisperse chitosan iron salt mixture hydrosol with a concentration of 0.09mol / L. Pour the obtained hydrosol into a special pear-shaped separating funnel ( figure 1 ), and then drop the hydrosol into 0.5mol / L NaOH solution drop by drop to prepare black transparent magnetic chitosan gel balls. After the dropwise addition, let the magnetic chitosan gel ball continue to stir in the lye for 1 hour, let it stand for 0.5 hour, filter and wash it to neutrality with ultrapure water. Finally, the wet gel balls were placed in a lyophilized vacuum dryer (-86° C., 1 Pa) and freeze-dried for 24...

Embodiment 2

[0046] A kind of preparation method of magnetic chitosan gel ball, carries out according to the following steps:

[0047] Add 3g chitosan in 100mL 0.5% acetic acid solution, make 3% chitosan solution, ferric salt (FeCl 3 ·6H 2 O) and ferrous salt (FeCl 2 4H 2 O) add in the chitosan solution according to the ratio of molar ratio 2:1, stir and make it mix uniformly, obtain Fe-containing 3 o 4 A monodisperse chitosan iron salt mixture hydrosol with a concentration of 0.11mol / L. Pour the obtained hydrosol into a special pear-shaped separating funnel ( figure 1 ), and then drop the hydrosol into 0.8mol / L NaOH solution drop by drop to prepare black transparent magnetic chitosan gel balls. After the dropwise addition, let the magnetic chitosan gel ball continue to stir in the lye for 2h, let it stand for 0.5h, filter and wash it to neutral with ultrapure water. Finally, the wet gel balls were placed in a lyophilized vacuum dryer (-86° C., 1 Pa) and freeze-dried for 24 hours to...

Embodiment 3

[0049] A kind of preparation method of magnetic chitosan gel ball, carries out according to the following steps:

[0050] Add 3g chitosan in 100mL 1% acetic acid solution, make 3% chitosan solution, ferric salt (FeCl 3 ·6H 2 O) and ferrous salt (FeCl 2 4H 2 O) add in the chitosan solution according to the ratio of molar ratio 2:1, stir and make it mix uniformly, obtain Fe-containing 3 o 4 A monodisperse chitosan iron salt mixture hydrosol with a concentration of 0.07mol / L. Pour the obtained hydrosol into a special pear-shaped separating funnel ( figure 1 ), and then drop the hydrosol into 0.8mol / L NaOH solution drop by drop to prepare black transparent magnetic chitosan gel balls. After the dropwise addition, let the magnetic chitosan gel ball continue to stir in the lye for 2 hours, let it stand for 1 hour, filter and wash it to neutrality with ultrapure water. Finally, the wet gel balls were placed in a lyophilized vacuum dryer (-86° C., 1 Pa) and freeze-dried for 24 ...

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Abstract

The invention discloses a method for immobilizing anaerobic iron ammonia oxidizing bacteria and treating ammonia-nitrogen wastewater through magnetic chitosan gel beads, particularly relates to a preparing method of the magnetic chitosan gel beads, a method for immobilizing the anaerobic iron ammonia oxidizing bacteria, and a method for treating ammonia nitrogen in the wastewater through the anaerobic iron ammonia oxidizing bacteria, and belongs to the field of wastewater treating preparation. The magnetic chitosan gel beads are prepared through a coprecipitation method, then the anaerobic iron ammonia oxidizing bacteria are fixed to the magnetic chitosan gel beads in an adsorbed mode through an adsorption method. The magnetic chitosan gel beads prepared through the method have double properties of a high polymer material and magnetic particles and have the good biological adsorption property; the anaerobic iron ammonia oxidizing bacteria can be fixed to the magnetic chitosan gel beads, the bacterial density is increased, and the ability to withstand adverse conditions is improved, so that the efficiency of removing the ammonia nitrogen by the anaerobic iron ammonia oxidizing bacteria is improved, and a new technology is provided for removing ammonia nitrogen in the wastewater.

Description

technical field [0001] The invention relates to the technical field of wastewater treatment, in particular to a method for immobilizing anaerobic Feammox bacteria by using magnetic chitosan gel balls. Background technique [0002] Ammonia nitrogen is a nutrient and the main oxygen-consuming pollutant in water bodies. If a large amount of ammonia nitrogen is discharged into water bodies, it will easily lead to eutrophication of water bodies and seriously endanger the health of water body ecosystems and drinking water sources. According to the 2016 China Environmental Status Bulletin, from the monitoring data of industrial pollution sources, domestic pollution sources, and comprehensive sewage outlets, the total amount of sewage discharge in my country is about 6,574.3 million tons, including 15,304 tons of ammonia nitrogen and 64,466 tons of total nitrogen; The nutritional status of lakes (reservoirs) was monitored, of which 90.8% were moderately nutritious and above, an incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10
CPCC12N11/10
Inventor 陈琛彭晓春刘志文桑燕鸿洪鸿加欧英娟汪元南廖树妹吴彦瑜
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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