Method for immobilizing anaerobic iron ammonia oxidizing bacteria through magnetic chitosan gel beads
A technology of chitosan gel balls and anaerobic iron and ammonia, applied in the direction fixed on/in the organic carrier, can solve the problems of river hazards, improve the living environment, improve the capacity, and improve the treatment efficiency Effect
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Embodiment 1
[0043] A kind of preparation method of magnetic chitosan gel ball, carries out according to the following steps:
[0044] Add 2g chitosan in 100mL 1% acetic acid solution, make 2% chitosan solution, ferric salt (FeCl 3 ·6H 2 O) and ferrous salt (FeCl 2 4H 2 O) add in the chitosan solution according to the ratio of molar ratio 2:1, stir and make it mix uniformly, obtain Fe-containing 3 o 4 A monodisperse chitosan iron salt mixture hydrosol with a concentration of 0.09mol / L. Pour the obtained hydrosol into a special pear-shaped separating funnel ( figure 1 ), and then drop the hydrosol into 0.5mol / L NaOH solution drop by drop to prepare black transparent magnetic chitosan gel balls. After the dropwise addition, let the magnetic chitosan gel ball continue to stir in the lye for 1 hour, let it stand for 0.5 hour, filter and wash it to neutrality with ultrapure water. Finally, the wet gel balls were placed in a lyophilized vacuum dryer (-86° C., 1 Pa) and freeze-dried for 24...
Embodiment 2
[0046] A kind of preparation method of magnetic chitosan gel ball, carries out according to the following steps:
[0047] Add 3g chitosan in 100mL 0.5% acetic acid solution, make 3% chitosan solution, ferric salt (FeCl 3 ·6H 2 O) and ferrous salt (FeCl 2 4H 2 O) add in the chitosan solution according to the ratio of molar ratio 2:1, stir and make it mix uniformly, obtain Fe-containing 3 o 4 A monodisperse chitosan iron salt mixture hydrosol with a concentration of 0.11mol / L. Pour the obtained hydrosol into a special pear-shaped separating funnel ( figure 1 ), and then drop the hydrosol into 0.8mol / L NaOH solution drop by drop to prepare black transparent magnetic chitosan gel balls. After the dropwise addition, let the magnetic chitosan gel ball continue to stir in the lye for 2h, let it stand for 0.5h, filter and wash it to neutral with ultrapure water. Finally, the wet gel balls were placed in a lyophilized vacuum dryer (-86° C., 1 Pa) and freeze-dried for 24 hours to...
Embodiment 3
[0049] A kind of preparation method of magnetic chitosan gel ball, carries out according to the following steps:
[0050] Add 3g chitosan in 100mL 1% acetic acid solution, make 3% chitosan solution, ferric salt (FeCl 3 ·6H 2 O) and ferrous salt (FeCl 2 4H 2 O) add in the chitosan solution according to the ratio of molar ratio 2:1, stir and make it mix uniformly, obtain Fe-containing 3 o 4 A monodisperse chitosan iron salt mixture hydrosol with a concentration of 0.07mol / L. Pour the obtained hydrosol into a special pear-shaped separating funnel ( figure 1 ), and then drop the hydrosol into 0.8mol / L NaOH solution drop by drop to prepare black transparent magnetic chitosan gel balls. After the dropwise addition, let the magnetic chitosan gel ball continue to stir in the lye for 2 hours, let it stand for 1 hour, filter and wash it to neutrality with ultrapure water. Finally, the wet gel balls were placed in a lyophilized vacuum dryer (-86° C., 1 Pa) and freeze-dried for 24 ...
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