Method for breeding medicinal plant valeriana jatamansi jones by means of tissue culture

Abstract

The invention discloses a method for breeding a medicinal plant valeriana jatamansi jones by means of tissue culture. The method includes steps of S1, selecting finely grown and robust wild valerianajatamansi jones, placing the wild valeriana jatamansi jones under tap water, flushing the wild valeriana jatamansi jones for more than 1 hour, then sufficiently immersing explants in 75% alcohol on anultraclean worktable in a sterile room, treating the explants for 30 s, flushing the explants by the aid of sterile water by 4-5 times, sufficiently soaking the explants in 0.1% mercury chloride solution for 10-15 min, repeatedly flushing the explants by the aid of sterile water by 5-6 times, then repeatedly shaking the explants to thoroughly clear mercury chloride, drying redundant moisture by the aid of sterile filter paper and then reserving the explants. The method for breeding the medicinal plant valeriana jatamansi jones by means of tissue culture has the advantages that the method includes simple culture steps and is easy to operate, and the medicinal plant valeriana jatamansi jones can be conveniently bred by staffs by means of tissue culture; callus tissues are induced and cultured, multiple experiments are compared to one another, accordingly, the optimal breeding conditions can be conveniently screened by users, and the requirements on industrially breeding the medicinal plant valeriana jatamansi jones by means of tissue culture can be met.

Description

technical field [0001] The invention relates to the technical field of cultivating the medicinal plant Sarmascaria, in particular to a method for tissue culture and breeding of the medicinal plant Sarmascens. Background technique [0002] Spider Incense ( Valeriana jatamansi Jones.), is a plant of the genus Valeriana in the family Pateriaceae, the dry rhizome and root of Spiderwort, also known as horseshoe incense, heart leaf valerian, tiger seven and other perennial herbaceous plants, 30-70cm high. Stems usually clustered in several branches, densely pubescent. The rhizome is horizontal, thick, massive, thick, with tight internodes, with petiole residues, yellowish brown, and has a specific aroma. It usually grows in damp forests in river valleys, open forest margins, river beaches, forests, roadside grass, hilltop meadows, and wet hillsides. The main areas of distribution are in Shaanxi Province, Hubei Province, Hunan Province, Chongqing City, Sichuan Province, Guangxi ...

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Problems solved by technology

[0004] Nowadays, the research on spider incense has attracted the attention of relevant industries, mainly focusing on the medicinal chemistry, pharmacological effects, volatile components, medicinal material quality standards and medicinal material quality analysis of spider incense, but few people have done the plant tissue culture of spider incense for the time being. According to related research, because of its wide range of uses and large amount of use, the wild resources are getting scarcer, and the contradiction between supply and demand is becoming increasingly prominent.

The current tissue culture and breeding of the medicinal plant Arachnida sinensis generally involves very complicated steps, and the requirements for plant body and cultivation conditions are relatively high during the cultivation process, so that the survival rate of Arachnid indica transplanted is very low, and the use is limited and cannot be used. Meet factory needs

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