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Application of recombinant porcine-source IL-6 to medicine preparing

A technology of IL-6 and uses, which is applied in the field of recombinant porcine-derived IL-6 in the field of pharmaceutical preparation, can solve the problems of unknown or uncertain regulation of porcine cytokines and the like

Inactive Publication Date: 2018-09-21
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the regulatory role of porcine cytokines in xenografting remains unknown or uncertain

Method used

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  • Application of recombinant porcine-source IL-6 to medicine preparing
  • Application of recombinant porcine-source IL-6 to medicine preparing
  • Application of recombinant porcine-source IL-6 to medicine preparing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] 1. Experimental materials

[0023] 1. Experimental equipment

[0024] 37°CCO 2 The cell incubator was purchased from Heraus, the ultra-clean bench was purchased from Sujing Group Ansen Company, the cell / particle counter was purchased from BECKMAN COULTER, Carl ZEISS fluorescence microscope, BIO-RAD protein electrophoresis instrument, flow cytometer BD, normal temperature and freezing Centrifuge Eppendorf, pure water device Millipore, Haier ultra-low temperature refrigerator, shaker ThermoFisher, real-time quantitative PCR instrument ABI 7900, automatic processor Danaford ML 400, and vacuum pump were purchased from Shanghai Jinghong.

[0025] 2. Primary Cells and Cell Lines

[0026] In the experiment, porcine aortic endothelial cells (PAECs), porcine peripheral blood mononuclear cells (PBMCs) and human umbilical vein endothelial cells (HUVECs) were mainly used. Among them, PBMCs and PAECs were isolated from wild-type Chinese Wuzhishan pig.

[0027] Human sera were obta...

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Abstract

The invention discloses application of recombinant porcine-source IL-6 to medicine preparing. The application of the recombinant porcine-source IL-6 comprises application to preparation of medicine for activating signal channels in human cells, application to preparation of medicine for up regulating expressions of adherence factors or inflammatory cell factors or tissue factors in human cells andapplication to preparation of medicine for regulating expressions of chemotactic factors in human cells; medicine for regulating signal channels or downstream gene expressions in human umbilical veinendothelial cells is provided based on the application, and an active ingredient in the medicine comprises the recombinant porcine-source IL-6. Through research findings, the recombinant porcine-source IL-6 can up regulate expressions of adherence factors ICAM-1, inflammatory cell factors IL-6, chemotactic factors MCP-1 and tissue factors, expressions of chemotactic factors IL-8 are reduced, andSTAT3 signal channels can be activated, and therefore the recombinant porcine-source IL-6 can serve as the active ingredient of the medicine capable of achieving the functions.

Description

technical field [0001] The application relates to the field of application of porcine-derived cytokines, in particular to the use of recombinant porcine-derived IL-6 in the preparation of medicines. Background technique [0002] Pro-inflammatory cytokines are produced in various human diseases such as cancer, autoimmune diseases, obesity and organ transplantation. These cytokines play an important role in inflammation in human diseases. Various proinflammatory cytokines are also produced in xenografts and cross-react with donor or recipient cells, and the inventors of the present application predict that they will play important roles in immune and coagulation responses. Many proinflammatory cytokines increase the expression of tissue factor (abbreviated TF), which promotes coagulation. [0003] Previously, the inventors of the present application reported that human IL-6, IL-17, IL-1β and TNF-α activated porcine aortic endothelial cells (abbreviated as PAECs) in different...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/20A61P29/00A61P37/06
CPCA61K38/204A61P29/00A61P37/06
Inventor 牟丽莎高汉超蔡志明
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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