Microbe sample preservation solution for gene detection, preparation method and kit thereof, and application of kit

Abstract

The invention provides a microbe sample preservation solution for gene detection, a preparation method and a kit thereof, and an application of the kit, and belongs to the technical field of bioengineering. The microbe sample preservation solution for gene detection has effective and comprehensive microbe inactivation capability; and low-concentration ethanol is used to thoroughly inactivate bacteria in the storage process of a sample, and avoids inactivation failure caused by the stress reaction of bacteria due to high-concentration ethanol. The microbe sample preservation solution has good permeability on the premise of not destroying the cell structure, and effectively destroys the protein activity of bacteria and viruses and the activities of various nucleases to make the bacteria, viruses and nucleases not have of infection or proliferation ability, so the thorough inactivation effect is achieved. A glycerol-containing cytoprotective agent and a physiological concentration buffersolution are used to keep the cell structure of microbes and prevent the cell structure from be ruptured in order to protect the integrity of nucleic acids in cells; and the microbe sample preservation solution contains no high-toxicity components or volatile components, so no volatile poisoning occurs during use.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a microbial sample preservation solution for gene detection, a preparation method, a kit and an application thereof. Background technique [0002] At present, the oral / fecal bacterial preservation solutions on the market are mainly composed of ethanol / methanol / SDS, and are prepared into weakly alkaline isotonic solutions, which can kill the bacteria in the sample, but will cause damage to the cell structure, resulting in Cell contents are released and cannot be detected by immunological or other methods. At the same time, methanol\guanidine isothiocyanate in the preservation solution is volatile or highly toxic, and it is easy to cause volatilization during use and cause harm to operators. [0003] The existing preservation solution is inactivated with high-purity ethanol; some bacteria will have a stress response to high-concentration ethanol, resulting in a reduced inac...

AI Technical Summary

Problems solved by technology

[0002] At present, the oral / fecal bacterial preservation solutions on the market are mainly composed of ethanol / methanol / SDS, and are prepared into weakly alkaline isotonic solutions, which can kill the bacteria in the sample, but will cause damage to the cell structure, resulting in Cell contents are released and cannot be used for immunological or other methods of detection

At the same time, methanol\guanidine isothiocyanate in the preservation solution is volatile or highly toxic, and it is easy to cause volatilization during use and cause harm to operators

[0003] The existing preservation solution is inactivated with high-purity ethanol; some bacteria will have a stress response to high-concentration ethanol, resulting in a reduced inactivation effect; it contains protective nucleic acid components, but a large amount of surfactant in the preservation solution can also cause cell rupture , causing nucleic acid etc. to be in an exposed environment and easily degraded and destroyed; some inactivators are highly toxic, and operational errors during use will cause poisoning to users

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