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Sugarcane variety xanthomonas albilineans indoor resistance identification method

A technology for resistance identification and white streak disease, which is applied in the field of identification of sugarcane disease resistance and indoor resistance identification of white streak disease of sugarcane varieties, can solve the problems that the identification method of sugarcane white streak disease resistance has not been established, and save land resources and labor and material resources , easy to operate, and shorten the breeding period

Inactive Publication Date: 2018-09-04
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

However, an efficient and accurate identification method for sugarcane white streak disease resistance has not been established so far.

Method used

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  • Sugarcane variety xanthomonas albilineans indoor resistance identification method
  • Sugarcane variety xanthomonas albilineans indoor resistance identification method

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Experimental program
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Embodiment 1

[0024] Embodiment 1, a kind of indoor resistance identification method of white streak disease of sugarcane variety, identification steps are as follows:

[0025] 1. Suspension culture of sugarcane white streak pathogen

[0026] The pathogenic strain XAFN1 was isolated from white streak leaf samples of sugarcane variety Funong 3504 planted in the sugarcane area of ​​Zhanjiang, Guangdong. After the strain was activated twice on the XAS medium by streaking on the plate, a single colony was selected and cultured on a shaker at 28°C and 200 rpm for 12 hours in the XAL medium, and the bacterial cells were collected, and the concentration of the bacterial solution was adjusted with sterile water. for 10 8 CFU / mL suspension of sugarcane white streak pathogen.

[0027] 2. Artificial planting of sugarcane seedlings

[0028] The stems of six different sugarcane varieties, including Ho95-988, LCP85-384, Xintaitang No. 22, Liucheng 09-15, and Yuegan No. 48, were selected and cut into ...

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Abstract

The invention relates to a sugarcane variety xanthomonas albilineans indoor resistance identification method. The method includes: bacterial suspension culture of sugarcane xanthomonas albilineans pathogenic bacteria, seedling cultivation of sugarcane variety or strain, inoculation identification and disease investigation. The method identifies the sugarcane variety xanthomonas albilineans resistance by means of leaf cutting inoculation, the method is close to the condition of artificially inoculated xanthomonas albilineans pathogenesis, and can indicate the resistance level of sugarcane variety to xanthomonas albilineans visually. Indoor inoculation is not disturbed by external environmental factors, has the characteristics of significant sugarcane variety incidence, good reproducibility,strong operability, simple operation, and low requirements for experimental equipment, and is convenient to carry out sugarcane variety xanthomonas albilineans resistance identification. The sugarcane variety xanthomonas albilineans indoor resistance identification method provided by the invention provides technical support for sugarcane xanthomonas albilineans resistance breeding, can quickly and accurately identify and evaluate the sugarcane variety xanthomonas albilineans resistance, saves and resources, labors and material resources, and greatly shortens the breeding period of xanthomonasalbilineans resistant sugarcane varieties.

Description

technical field [0001] The invention relates to a sugarcane disease resistance identification method, in particular to a sugarcane variety white stripe disease indoor resistance identification method, which belongs to the technical field of crop disease resistance identification. Background technique [0002] Sugarcane white streak disease is one of the three major sugarcane bacterial diseases in the world. It is caused by Xanthomonas spp. Xanthomonas albilineans ) lead to. In susceptible varieties, the vascular bundles of sugarcane stems are silted up by the mucus of the pathogen, which affects water transportation and causes striated necrosis of sugarcane leaves, which seriously affects the yield and quality of sugarcane. There are three forms of the disease, one is chronic symptoms: at the beginning of the disease, there are 1-2 mm wide white slender stripes parallel to the veins, the number and length of which are different, or slender, or the whole leaf turns white , ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/64
CPCC12Q1/18G01N2333/195
Inventor 高三基林岭虹林艺华西尔万张慧丽傅华英王锦达黄美婷孟建玉
Owner FUJIAN AGRI & FORESTRY UNIV
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