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Sugarcane top rot resistance identification method

A sugarcane tip rot and resistance identification technology is applied in the field of crop disease resistance identification, which can solve the problem of not establishing a sugarcane tip rot resistance identification method, etc., achieves low requirements for experimental equipment, reduces manpower and material resources, and is easy to operate. Effect

Inactive Publication Date: 2015-03-25
GUANGXI UNIV
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Problems solved by technology

[0004] However, an efficient and accurate identification method for sugarcane tip rot resistance has not been established so far. In view of the increasing trend of sugarcane tip rot in my country, a set of fast and effective identification methods for sugarcane tip rot resistance and screening of resistant germplasms have been established. Resources and Variety Imperatives

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  • Sugarcane top rot resistance identification method
  • Sugarcane top rot resistance identification method
  • Sugarcane top rot resistance identification method

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Embodiment 1

[0044] 1. Preparation of inoculum

[0045] The spore suspensions of sugarcane tip rot pathogen strain CNO~1 and strain YN41 (provided by the Laboratory of Innovation and Genetic Improvement of Sugarcane Germplasm Resources of Guangxi University) were prepared respectively. The preparation method of the spore suspension of the two strains is the same, specifically: inoculate the pathogenic bacteria of sugarcane tip rot on PDA medium to activate for 3 days, pick edge hyphae and inoculate them in sterilized potato dextrose water medium for shaking culture for 3 days , collected the bacteria by centrifugation, filtered with sterilized absorbent cotton, and adjusted the spore concentration to 1×10 with sterile water. 6 CFU / ml concentration. The spore suspension of strain CNO~1 and the spore suspension of strain YN41 were obtained.

[0046] 2. Treatment of inoculated materials

[0047] Select different sugarcane varieties as inoculation materials, including Xintaitang 22, Fugui N...

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Abstract

The invention discloses a sugarcane top rot resistance identification method. The method comprises the steps of injecting a well-prepared sugarcane top rot pathogenic bacterium spore suspension onto a growth point of a sugarcane seedling, setting blank sterile water as a reference, culturing the sugarcane seedling for 10 to 15 days after the sugarcane seedling is inoculated, investigating the pathogenic bacterium breeding situation, and classifying the resistance of the sugarcane variety to the sugarcane top rot into an immunity grade, a disease-resisting grade, a medium-resisting grade and an infectious grade according to the disease degree and the disease situation. The sugarcane seedling top rot is identified by adopting an injection inoculation way, the method is closer to the real situation that the sugarcane seedling in the field is infected with the top rot, and the resistance of the sugarcane seedlings of various varieties to the sugarcane top rot can be directly reflected.

Description

technical field [0001] The invention relates to a sugarcane tip rot resistance identification method, which belongs to the technical field of crop disease resistance identification. Background technique [0002] The pathogen of sugarcane tip rot (Pokkah Boeng Disease) is Fusarium moniliformae (Fusarium moniliformae), which can produce beaded conidia or pseudocephalic sporulation in the asexual stage, and its sexual form is Gibberella moniliformis. Sugarcane tip rot mainly occurs on the young leaves and tips of sugarcane. After being infected, it causes the tip of the plant to rot, hence the name tip rot. After infection, the heart leaves are trapezoidal, concavo-convex and distorted, with longitudinal fissures, and the leaves at the tips are often entangled and deformed, and obvious brown wrinkles appear. When the plant is severely damaged, the tissue around the growth point will become soft and brown, the heart and leaves will be necrotic, forming tip rot, resulting in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18A01G7/00
Inventor 张木清徐世强王继华
Owner GUANGXI UNIV
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