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Compound microorganism deodorization fungicide as well as preparation method and application thereof

A technology for compounding microorganisms and deodorizing bacteria agents, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of non-reusability, easy saturation of adsorbents, and reduced deodorization effect, so as to reduce odor. Release strength, stable quality, and small batch-to-batch variation

Active Publication Date: 2018-08-28
上海华浩原益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method absorbs the odorous substances into the porous carrier through the adsorbent. The adsorbent is easy to be saturated and difficult to desorb, resulting in the inability to be reused and causing secondary pollution to the environment; chemical deodorants are highly targeted and quick-acting. However, because of its uniqueness in removing odorous gases, when it encounters complex odorous gas components, its deodorizing effect will be greatly reduced, and at the same time, the cost of use is high, and it is easy to cause secondary pollution to the environment; microbial deodorants are used Exogenous functional bacteria or biological enzymes can directly absorb and degrade odor components or inhibit odor-producing microorganisms to remove odor

Method used

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  • Compound microorganism deodorization fungicide as well as preparation method and application thereof
  • Compound microorganism deodorization fungicide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Concrete, embodiment 1: the preparation of composite microbial deodorant bacterium agent, its steps are as follows:

[0038] (1) Configure YPD and MRS solid medium. The formula of YPD solid medium is peptone 20g, beef extract 10g, glucose 20g, water 1000mL, agar 15-20g, pH6.5-7.0; MRS solid medium formula is peptone 10g , beef extract 10g, yeast extract 5g, glucose 5g, sodium acetate 5g, diammonium citrate 2g, Tween 801mL, magnesium sulfate 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, agar 15-20g, water 1000mL, pH6.2; beef extract peptone solid medium is 5g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar, 1000mL water, pH7.0-7.5. After configuration, the culture medium was sterilized at 121°C for 20 minutes;

[0039] (2) Injecting Hansenula anomalies and Candida quaternidae into YPD medium for activation; inserting Lactobacillus plantarum and Lactobacillus acidophilus into MRS medium for activation respectively;

[0040] (3) Configure ...

Embodiment 2

[0043] Embodiment 2: comparative experiment

[0044] Prepare the microbial agent according to the method of Example 1, wherein the ratio of the fermentation broth of the agent is: Hansenula wickii: Candida quaternidae: Lactobacillus plantarum: Lactobacillus acidophilus = 1:1:2:2 (vol / vol) mix. In addition, the fermentation liquid of four kinds of bacteria was used as deodorizing treatment agent alone for comparative verification.

[0045] Put 20kg of domestic garbage from the garbage compression station into six 100L sealed plastic boxes, and spray 10mL of the five deodorants prepared above with an atomizer from the top every day. As a control, it was sprayed continuously for 5 days, and the odor intensity, ammonia gas concentration and hydrogen sulfide concentration were measured after 10 days.

[0046] The measurement results are shown in the table below:

[0047] group

[0048] It can be seen from the above table that after adding 5 kinds of liquid microbial de...

Embodiment 3

[0049] Embodiment 3: the application of composite microbial bacterial agent

[0050] A large-scale farm was selected in Sihong County, Jiangsu Province, with a stock of 5,000 pigs and a construction area of ​​about 200m 2 pig manure composting field, select 100m of them 2 Sealed with plastic film as the experimental group, another 100m 2 for the control group. The experimental group was sprayed with 30kg of complex bacterial agent fermentation liquid every day for 5 consecutive days. After 10 days, the ammonia gas concentration and hydrogen sulfide concentration of the two groups were measured. The results are shown in the table below.

[0051]

[0052]

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Abstract

The invention relates to a compound microorganism deodorization fungicide used for waste compression stations, waste transfer stations, public toilets and livestock and poultry farms. The microorganism deodorization fungicide contains abnormal Wacker hansenula polymorpha of which the biological preservation number is ATCC (American Type Culture Collection) 18205, Guilliermond candida of which thebiological preservation number is ACCC (Agricultural Culture Collection of China) 20232, plant lactobacillus of which the biological preservation number is ACCC 10241 and lactobacillus acidophilus ofwhich the biological preservation number is ACCC 00159. The microorganism deodorization fungicide can inhibit a situation that organic matters are subjected to putrefactive fermentation to generate malodorous gas, meanwhile, malodorous substances including NH3, ammonium sulfate, methyl mercaptan and the like can be adsorbed and utilized, and the compound microorganism deodorization fungicide has an obvious deodorization effect. The invention also discloses a preparation method for the microorganism deodorization fungicide, and the application of the microorganism deodorization fungicide.

Description

technical field [0001] The invention relates to a compound microbial deodorizing agent used in places such as garbage compression stations, garbage transfer stations, public toilets and livestock and poultry farms, as well as its preparation method and application. Background technique [0002] Along with social progress and economic development, while people's quality of life is constantly improving, their environmental awareness is also increasing, and the control and treatment of malodorous gases has been paid more and more attention. The malodorous gas mainly contains some sulfur-containing compounds and nitrogen-containing compounds, such as hydrogen sulfide, methyl mercaptan, sulfide, ammonia, etc., which have a strong irritating odor and can enter the human body through different channels such as the respiratory tract, eyes, and skin, making people Dizziness, discomfort, and being in it for a long time will do great damage to the nervous system of the human body. [...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F11/02C12R1/645C12R1/72C12R1/23C12R1/25C02F101/10C02F101/16
CPCC12N1/16C12N1/20C02F11/02C02F2101/16C02F2101/101
Inventor 孙浩议李登奎王斐朱亭
Owner 上海华浩原益生物科技有限公司
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