Plant growth regulator for improving stress resistance and application thereof
A plant growth regulator, plant growth regulation technology, applied in the direction of plant growth regulators, plant growth regulators, biocides, etc., can solve the problems of unpredictable resistance to biotic adversity and abiotic adversity, and achieve excellent thermal stability sex, no side effects, safe side effects
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Embodiment 1
[0041] Embodiment one, prepare plant growth regulator by Lactobacillus paracasei GMNL-32
[0042] First, this example evaluates the mycological characteristics of Lactobacillus paracasei (GMNL-32).
[0043] 1. Mycological characteristics of Lactobacillus paracasei GMNL-32
[0044] The source of Lactobacillus paracasei (Lactobacillus paracasei) GMNL-32 is the gastrointestinal tract of healthy human beings. Its appearance and physiological characteristics have been disclosed in Chinese Patent Publication No. CN 100396771C, which is hereby incorporated as a reference.
[0045] Secondly, Lactobacillus paracasei GMNL-32 was confirmed as Lactobacillus paracasei after being analyzed by API 50CHI V5.1 identification kit (figure not shown).
[0046] In addition, after the total RNA of Lactobacillus paracasei GMNL-32 was extracted using known methods, the 16S was amplified by a primer pair such as sequence number (SEQID NO): 1 and 2 (the upstream primer is a PAF primer, and the downstr...
Embodiment 2
[0051] Embodiment two, assessing the effect of plant growth regulators on improving plant resistance to adversity
[0052] 1. Evaluate the effect of plant growth regulators on improving the resistance of tomato plants to biological stress 1.1 Inoculation of plant pathogenic bacteria
[0053] This embodiment utilizes tomato plant (Solanum lycopersicum, line: Nongyou 301, Nongyou Seedling Company, Taiwan), 4 to 5 weeks old), and its leaves are first fermented by Lactobacillus paracasei GMNL-32 of embodiment one in the field After the culture liquid or the heat-killed fermentation liquid of Lactobacillus paracasei GMNL-32 was treated for 11 times, the following plant pathogenic bacteria were inoculated respectively.
[0054] Colletotrichum gloeosporioides (Colletotrichum gloeosporioides; causing tomato anthracnose) was cultured in 1 / 5 potato-dextrose-agar medium (Potato Dextrose Agar; PDA) medium, with 5 mL of 0.1% water agar containing 0.05% Tween 20 ( water agar; WA) After the...
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